Simultaneous Assessment of Acidogenesis-Mitigation and Specific Bacterial Growth-Inhibition by Dentifrices

Article, Review English OPEN
Forbes, Sarah ; Latimer, Joe ; Sreenivasan, Prem K. ; McBain, Andrew J. (2016)
  • Publisher: Public Library of Science
  • Journal: PLoS ONE, volume 11, issue 2 (issn: 1932-6203, eissn: 1932-6203)
  • Related identifiers: doi: 10.1371/journal.pone.0149390, pmc: PMC4755603
  • Subject: Microbial Control | Research Article | Bacteria | Anaerobic Bacteria | Anatomy | Fluorides | Infectious Diseases | Antimicrobials | Chemical Compounds | Physical Sciences | Microbial Physiology | Pharmacology | Chemistry | Oral Medicine | Biology and Life Sciences | Drugs | Developmental Biology | Microbial Growth and Development | Microbiology | Physiology | Medicine | Body Fluids | Bacterial Growth | Saliva | Bacterial Diseases | Q | R | Oral Diseases | Science | Organisms | Medicine and Health Sciences | Caries

Dentifrices can augment oral hygiene by inactivating bacteria and at sub-lethal concentrations may affect bacterial metabolism, potentially inhibiting acidogenesis, the main cause of caries. Reported herein is the development of a rapid method to simultaneously measure group-specific bactericidal and acidogenesis-mitigation effects of dentifrices on oral bacteria. Saliva was incubated aerobically and anaerobically in Tryptone Soya Broth, Wilkins-Chalgren Broth with mucin, or artificial saliva and was exposed to dentifrices containing triclosan/copolymer (TD); sodium fluoride (FD); stannous fluoride and zinc lactate (SFD1); or stannous fluoride, zinc lactate and stannous chloride (SFD2). Minimum inhibitory concentrations (MIC) were determined turbidometrically whilst group-specific minimum bactericidal concentrations (MBC) were assessed using growth media and conditions selective for total aerobes, total anaerobes, streptococci and Gram-negative anaerobes. Minimum acid neutralization concentration (MNC) was defined as the lowest concentration of dentifrice at which acidification was inhibited. Differences between MIC and MNC were calculated and normalized with respect to MIC to derive the combined inhibitory and neutralizing capacity (CINC), a cumulative measure of acidogenesis-mitigation and growth inhibition. The overall rank order for growth inhibition potency (MIC) under aerobic and anaerobic conditions was: TD> SFD2> SFD1> FD. Acidogenesis-mitigation (MNC) was ordered; TD> FD> SFD2> SFD1. CINC was ordered TD> FD> SFD2> SFD1 aerobically and TD> FD> SFD1> SFD2 anaerobically. With respect to group-specific bactericidal activity, TD generally exhibited the greatest potency, particularly against total aerobes, total anaerobes and streptococci. This approach enables the rapid simultaneous evaluation of acidity mitigation, growth inhibition and specific antimicrobial activity by dentifrices.
  • References (33)
    33 references, page 1 of 4

    1. Aas JA, Paster BJ, Stokes LN, Olsen I, Dewhirst FE. Defining the normal bacterial flora of the oral cavity. J Clin Microbiol. 2005; 43: 5721-5732. PMID: 16272510

    2. Kornman KS. The role of supragingival plaque in the prevention and treatment of periodontal diseases. J Periodont Res. 1986; 21: 5.

    3. Marsh PD. Microbial ecology of dental plaque and its significance in health and disease. Adv Dent Res. 1994; 8: 263-271. PMID: 7865085

    4. Touger-Decker R, Van Loveren C. Sugars and dental caries. Am J Clin Nutr. 2003; 78: 881S-892S. PMID: 14522753

    5. Woodward M, Walker A. Sugar consumption and dental caries: evidence from 90 countries. Br Dent J. 1994; 176: 297-302. PMID: 8186040

    6. Giacaman RA, Araneda E, Padilla C. Association between biofilm-forming isolates of mutans streptococci and caries experience in adults. Arch Oral Biol. 2010; 44: 550-554.

    7. Newbrun E. Sugar and dental caries: A review of human studies. Science. 1982; 217: 418. PMID: 7046052

    8. Axelsson P, Nystrm B, Lindhe J. The longterm effect of a plaque control program on tooth mortality, caries and periodontal disease in adults. J Clin Periodontol. 2004; 31: 749-757.

    9. Dong YM, Pearce E, Yue L, Larsen M, Gao XJ, Wang JD. Plaque pH and associated parameters in relation to caries. Caries Res. 1999; 33: 428-436. PMID: 10529527

    10. Van Houte J, Sansone C, Joshipura K, Kent R. Mutans streptococci and non-mutans streptococci acidogenic at low pH, and in vitro acidogenic potential of dental plaque in two different areas of the human dentition. J Dent Res. 1991; 70: 1503-1507. PMID: 1774381

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