Travelling-wave ion mobility mass spectrometry and negative ion fragmentation of hybrid and complex N-glycans

Article, Other literature type English OPEN
Harvey, David J. ; Scarff, Charlotte A. ; Edgeworth, Matthew ; Pagel, Kevin ; Thalassinos, Konstantinos ; Struwe, Weston B. ; Crispin, Max ; Scrivens, Jim (2016)
  • Related identifiers: doi: 10.1002/jms.3828
  • Subject: CID, N-linked carbohydrates, T-wave ion mobility, complex N-glycans, hybrid N-glycans, isomers, negative ion | Article

Nitrogen collisional cross sections (CCSs) of hybrid and complex glycans released from the glycoproteins IgG, gp120 (from human immunodeficiency virus), ovalbumin, α1-acid glycoprotein and thyroglobulin were measured with a travelling-wave ion mobility mass spectrometer using dextran as the calibrant. The utility of this instrument for isomer separation was also investigated. Some isomers, such as Man3 GlcNAc3 from chicken ovalbumin and Man3 GlcNAc3 Fuc1 from thyroglobulin could be partially resolved and identified by their negative ion fragmentation spectra obtained by collision-induced decomposition (CID). Several other larger glycans, however, although existing as isomers, produced only asymmetric rather than separated arrival time distributions (ATDs). Nevertheless, in these cases, isomers could often be detected by plotting extracted fragment ATDs of diagnostic fragment ions from the negative ion CID spectra obtained in the transfer cell of the Waters Synapt mass spectrometer. Coincidence in the drift times of all fragment ions with an asymmetric ATD profile in this work, and in the related earlier paper on high-mannose glycans, usually suggested that separations were because of conformers or anomers, whereas symmetrical ATDs of fragments showing differences in drift times indicated isomer separation. Although some significant differences in CCSs were found for the smaller isomeric glycans, the differences found for the larger compounds were usually too small to be analytically useful. Possible correlations between CCSs and structural types were also investigated, and it was found that complex glycans tended to have slightly smaller CCSs than high-mannose glycans of comparable molecular weight. In addition, biantennary glycans containing a core fucose and/or a bisecting GlcNAc residue fell on different mobility-m/z trend lines to those glycans not so substituted with both of these substituents contributing to larger CCSs. Copyright © 2016 John Wiley & Sons, Ltd.
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    1) Oxford Glycobiology Institute, Department of Biochemistry, University of Oxford, South Parks Road, Oxford, OX1 3QU, UK. 2) Department of Biological Sciences, University of Warwick, Coventry, CV47AL, UK.

    3) Current address, Astbury Centre for Structural Molecular Biology, School of Molecular and Cellular Biology, University of Leeds, Leeds, LS2 9JT, UK.

    4) Current address, MedImmune, Sir Aaron Klug Building, Granta Park, Cambridge, CB21 6GH, UK 5) Institute of Chemistry and Biochemistry, Freie Universität Berlin, Takustrasse. 3, 14159 Berlin, Germany.

    6) Institute of Structural and Molecular Biology, Department of Biological Sciences, Birkbeck College, University of London, London, WC1E 7HX, UK.

    Communications: Dr David J. Harvey, Oxford Glycobiology Institute, Department of Biochemistry, South Parks Road, Oxford, OX1 3QU, UK.

    Tel. (44) (0) 1865 275750 Fax. (44) (0) 1865 275216 e-mail david.harvey@bioch.ox.ac.uk

    [23] Harvey DJ, Scrivens JH, Holland R, Williams JP, Wormald MR. Ion-mobility separation coupled with negative ion fragmentation of N-linked carbohydrates. Paper presented at the 56th ASMS Conference on Mass Spectrometry, Denver, 2008: Proceedings CD MOG 09.10.

    [24] Hermannová M, Iordache Adistributions of product ions reveal isomeric ratio of deprotonated molecules in ion mobility mass spectrometry of hyaluronan-derived oligosaccharides. J. Mass Spectrom. 2015; 50: 854 863.

    [25] Harvey DJ, Abrahams JL. Fragmentation and ion mobility properties of negative ions from Nlinked carbohydrates: Part 7: Reduced glycans. Rapid Commun. Mass Spectrom. 2016; 30: 627-634.

    [26] Harvey DJ, Sobott F, Crispin M, Wrobel A, Bonomelli C, Vasiljevic S, Scanlan CN, Scarff C, Thalassinos K, Scrivens JH. Ion mobility mass spectrometry for extracting spectra of Nglycans directly from incubation mixtures following glycan release: Application to glycans from engineered glycoforms of intact, folded HIV gp120. J. Am. Soc. Mass Spectrom. 2011; 22: 568-581.

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