Isolation of digital dermatitis treponemes from cattle hock skin lesions

Article English OPEN
Clegg, S R ; Bell, J ; Ainsworth, S ; Blowey, R W ; Bell, N J ; Carter, S. D ; Evans, N J
  • Publisher: Blackwell Publishing Ltd
  • Related identifiers: doi: 10.1111/vde.12286
  • Subject: C522 Veterinary Microbiology | D420 Livestock | D328 Animal Welfare

Background: Bovine hock lesions present a serious welfare and production issue on dairy farms worldwide. Current theories suggest that trauma is an important factor in the formation of hock lesions, although infection may also play a role in increasing their severity and duration. Hypothesis: Digital dermatitis (DD) lesions in dairy cows are strongly associated with specific treponeme bacteria which are opportunistic invaders of other skin regions. Hock lesions were tested to ascertain if they too contained treponemes. Animals: Swab and tissue samples were taken from hock lesions from two farms in South West England. Methods: Hock lesions were classified into two categories: open lesions, which were often bleeding and ulcerated, or were encrusted; and closed lesions, which were classified as hair loss with no skin breakage. PCR assays and bacterial isolation were used to detect treponemes in hock lesions. Results: All three phylogroups of digital dermatitis treponemes were detectable and isolated from open hock lesions only, with closed lesions showing no evidence of treponeme infection, either by PCR or bacterial culture. When analysed by 16S rRNA gene sequencing, the cultured treponeme DNA showed complete homology or was very similar to that found in foot lesions. Additionally, skin swabs from near the open hock wounds were also positive by PCR assay and isolation for the DD treponemes. Conclusions and clinical importance: Identification of the contribution of these infectious agents will allow for more optimal treatments to be developed that reduce the prevalence and healing times of both hock and DD lesions. © 2016 ESVD and ACVD.
  • References (27)
    27 references, page 1 of 3

    Dhawi, A., Hart, C. A., Demirkan, I., Davies, I. H., Carter, S. D. (2005) Bovine digital dermatitis and severe virulent ovine foot rot: a common spirochaetal pathogenesis.

    Veterinary Journal. 169. 232-41.

    Dawson, J. C. (1998) Digital dermatitis - survey and debate. Proceedings of the XXth World Buiatrics Congress, Sydney. pp 91-93 Demirkan, I., S. D. Carter, C. Winstanley, K. D. Bruce, N. M. McNair, M. Woodside, and C.

    A. Hart. 2001. Isolation and characterisation of a novel spirochaete from severe virulent ovine foot rot. J. Med. Microbiol. 50:1061-1068.

    Dillon, M.B. (2011). Skin as a potential source of infectious foot and mouth disease aerosols.

    Proceedings of the Royal Society B. 278. 1761-1769 Duncan, J.S., Angell, J. W., Carter, S. D., Evans, N. J., Sullivan, L. E., Grove-White, D. H.

    (2014) Contagious ovine digital dermatitis: An emerging disease. Veterinary Journal. 201.

    Edwards, A. M, Dymock, D., Woodward, M. J., Jenkinson, H. F. (2003) Genetic relatedness and phenotypic characteristics of Treponema associated with human periodontal tissues and ruminant foot disease. Microbiology. 149. 1083-93 Espejo, L.A., Endres, M.I., Salfer, J.A., 2006. Prevalence of lameness in high-producing Holstein cows housed in freestall barns in Minnesota. Journal of Dairy Science 89, 3052- 3058.

    A., Carter, S.D. (2008). Three unique groups of spirochetes isolated from digital dermatitis lesions in UK cattle. Veterinary Microbiology. 130. 141-150.

    Evans, N. J., Brown, J. M., Demirkan, I., Singh, P., Getty, B., Timofte, D., Vink, W. D., Murray, R. D., Blowey, R. W., Birtles, R. J., Hart, C. A., Carter, S. D., (2009). Association of unique, isolated treponemes with bovine digital dermatitis lesions. J. Clin. Micro. 47, 689-696 Evans, N.J, Brown, J. M, Demirkan, I., Murray, R.D, Birtles, R.J., Hart, C. A., and Carter, S.

  • Metrics
    No metrics available
Share - Bookmark