Effect of diet type on serum and faecal concentration of S100/calgranulins in the captive cheetah

Article English OPEN
Depauw, S ; Heilmann, RM ; Whitehouse-Tedd, K ; Hesta, M ; Steiner, JM ; Suchodolski, JS ; Janssens, GPJ (2014)
  • Publisher: European Association of Zoos and Aquaria
  • Subject:
    mesheuropmc: fluids and secretions

Gastrointestinal disease is omnipresent in captive cheetahs (Acinonyx jubatus), in contrast to its freeranging populations. The current study aimed to evaluate the effect of diet type (meat-only whole prey) on gastrointestinal health in captive cheetahs by measuring faecal and serum concentrations of S100/calgranulins. This paper reports faecal S100A12 and calprotectin concentrations in 12 captive cheetahs fed supplemented beef versus whole rabbit for one month in a cross-over design. Also, serum S100A12 and calprotectin concentrations were determined in four cheetahs fed whole rabbit and six cheetahs fed supplemented beef, and these were compared to the faecal concentrations of the respective marker proteins. Both the immunoassay for canine calprotectin and canine S100A12 were precise and reproducible for use with serum samples and faecal extracts. Whereas the assay for S100A12 was linear and accurate, an inconsistent linearity of the canine calprotectin assay was observed and could be indicative of an insufficient cross-reactivity of the specific antibody used for this assay. Serum concentrations of S100A12 and calprotectin were not altered by diet type, and were not correlated with the respective concentrations in faeces. Numerically (P=0.241) greater calprotectin concentrations and greater (P=0.041) faecal S100A12 concentrations were detected in cheetahs fed supplemented beef compared with whole rabbit. These findings demonstrate that whole prey feeding may decrease intestinal inflammation in the captive cheetah. Consequently, the relation between diet type and intestinal inflammatory conditions in the captive cheetah warrants further investigation.
  • References (33)
    33 references, page 1 of 4

    Costa F., Mumolo M.G., Bellini M., Romano M.R., Ceccarelli L., Arpe P., Sterpi C., Marchi S., Maltinti G. (2003) Role of faecal calprotectin as non-invasive marker of intestinal inflammation. Digestive and Liver Disease 35: 642-647.

    Depauw S., Hesta M., Whitehouse-Tedd K., Vanhaecke L., Verbrugghe A., Janssens, G.P.J. (2011) Animal fibre: the forgoetn nutrient in strict carnivores? First insights in the cheetah. Journal of Animal Physiology and Animal Nutrition 97: 146-157.

    Depauw S., Bosch G., Hesta M., Whitehouse-Tedd K., Hendriks W., Kaandorp J., Janssens, G.P.J. (2012) Fermentation of animal components in strict carnivores: a comparative study with cheetah fecal inoculum. Journal of Animal Science 90: 2540-2548.

    D'Inca R., Dal Pont E., Di Leo V., Ferronato A., Fries W., Veotrato M.G., Martines D., Sturniolo G.C. (2008) Calprotectin and lactoferrin in the assessment of intestinal inflammation and organic disease. International Journal of Colorectal Disease 22: 429-437.

    Fagerholl M.K. (2000) Calprotectin, a faecal marker of organic gastrointestinal abnormality. The Lancet 356: 1783-1784.

    Foell D., Kucharzik T., Kraft M., Vogl T., Sorg C., Domschke W., Roth J. (2003) Neutrophil derived human S100A12 (EN-RAGE) is strongly expressed during chronic acute active inflammatory bowel disease. Gut 52: 847-853.

    Foell D., Witkowski H., Roth J. (2009) Monitoring disease activity by stool analyses: from occult blood to molecular markers of intestinal inflammation and damage. Gut 58: 859-868.

    Grellet A., Heilmann R.M., Feugier A., Lecoindre P., Hernandez J., Freiche V., Peeters D., Suchodolski J.S., Steiner J.M. (2011) Fecal calprotectin concentration in adult dogs with acute and chronic gastrointestinal disease. Journal of Veterinary Internal Medicine 25: 1486-1487 (abstract).

    Heilmann R.M., Suchodolski J.S., Steiner J.M. (2008) Development and analytic validation of a radioimmunoassay for the quantifcation of canine calprotectin in serum and feces from dogs. American Journal of Veterinary Research 69: 845-853.

    Heilmann R.M., Suchodolski J.S., Steiner J.M. (2011a) Development and analytic validation of an immunoassay for the quantifcation of canine S100A12 in serum and fecal samples and its biological variability in serum from healthy dogs. Veterinary Immunology and Immunopathology 144: 200-209.

  • Metrics
    views in OpenAIRE
    views in local repository
    downloads in local repository

    The information is available from the following content providers:

    From Number Of Views Number Of Downloads
    Institutional Repository - IRUS-UK 0 22
Share - Bookmark