The structure of O-polysaccharide isolated from Cronobacter universalis NCTC 9529T

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Marszewska, K ; Czerwicka, M ; Forsythe, SJ ; Sałdak, E ; Szulta, S ; Dziadziuszko, H ; Ossowska, K ; Kaczyński, Z (2014)

The O-polysaccharide (OPS) was isolated from Cronobacter universalis NCTC 9529T, a new species in the genus Cronobacter, which was created by the reclassification of the species Enterobacter sakazakii. Purified polysaccharide was analyzed by NMR spectroscopy (1H, COSY, TOCSY, ROESY, HSQC, and HSQC-TOCSY) and chemical methods. The monosaccharide derivatives were analyzed by gas chromatography, and gas chromatography-mass spectrometry. These experiments enabled the type and number of monosaccharides in the repeating unit of OPS, their positions of linkages, and absolute configuration to be determined. Together the chemical analysis established a structure of the OPS of C. universalis NCTC 9529T: \ud \ud →3)--L-FucpNAc-(1→4)--D-Manp-(1→3)--L-FucpNAc-(1→3)-β-D-GlcpNAc-(1→\ud \ud [A, B, C, D]\ud \ud OPS isolated from C. universalis was structurally characterized for the first time.
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