Characterisation of synovial fluid and infrapatellar fat pad derived mesenchymal stromal cells: The influence of tissue source and inflammatory stimulus

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Garcia, John ; Wright, Karina ; Roberts, Sally ; Kuiper, Jan Herman ; Mangham, Chas ; Richardson, James ; Mennan, Claire (2016)
  • Publisher: Nature Publishing Group
  • Journal: Scientific Reports, volume 6 (issn: 2045-2322, eissn: 2045-2322)
  • Related identifiers: doi: 10.1038/srep24295, pmc: PMC4829842
  • Subject: Article | R1

The infrapatellar fat pad (FP) and synovial fluid (SF) in the knee serve as reservoirs of mesenchymal stromal cells (MSCs) with potential therapeutic benefit. We determined the influence of the donor on the phenotype of donor matched FP and SF derived MSCs and examined their immunogenic and immunomodulatory properties before and after stimulation with the pro-inflammatory cytokine interferon-gamma (IFN-γ). Both cell populations were positive for MSC markers CD73, CD90 and CD105, and displayed multipotency. FP-MSCs had a significantly faster proliferation rate than SF-MSCs. CD14 positivity was seen in both FP-MSCs and SF-MSCs, and was positively correlated to donor age but only for SF-MSCs. Neither cell population was positive for the co-stimulatory markers CD40, CD80 and CD86, but both demonstrated increased levels of human leukocyte antigen-DR (HLA-DR) following IFN-γ stimulation. HLA-DR production was positively correlated with donor age for FP-MSCs but not SF-MSCs. The immunomodulatory molecule, HLA-G, was constitutively produced by both cell populations, unlike indoleamine 2, 3-dioxygenase which was only produced following IFN-γ stimulation. FP and SF are accessible cell sources which could be utilised in the treatment of cartilage injuries, either by transplantation following ex-vivo expansion or endogenous targeting and mobilisation of cells close to the site of injury.
  • References (58)
    58 references, page 1 of 6

    1. Brittberg, M. et al. Treatment of deep cartilage defects in the knee with autologous chondrocyte transplantation. N. Engl. J. Med. 331, 889-895 (1994).

    2. Bhosale, A. M., Kuiper, J. H., Johnson, W. E. B., Harrison, P. E. & Richardson, J. B. Midterm to long-term longitudinal outcome of autologous chondrocyte implantation in the knee joint: a multilevel analysis. Am. J. Sports Med. 37, 131S-138S (2009).

    3. LaPrade, R. F. & Botker, J. C. Donor-site morbidity aeftr osteochondral autograft transfer procedures. Arthroscopy 20, e69-73 (2004).

    4. Matricali, G. A., Dereymaeker, G. P. E. & Luyten, F. P. Donor site morbidity aeftr articular cartilage repair procedures: a review. Acta Orthop. Belg. 76, 669-674 (2010).

    5. Paul, J. et al. Donor-site morbidity aeftr osteochondral autologous transplantation for lesions of the talus. J. Bone Joint Surg. Am. 91, 1683-1688 (2009).

    6. Jones, E. A. et al. Enumeration and phenotypic characterization of synovial uflid multipotential mesenchymal progenitor cells in inflammatory and degenerative arthritis. Arthritis Rheum. 50, 817-827 (2004).

    7. Jones, E. A. et al. Synovial uflid mesenchymal stem cells in health and early osteoarthritis: detection and functional evaluation at the single-cell level. Arthritis Rheum. 58, 1731-1740 (2008).

    8. Sekiya, I. et al. Human mesenchymal stem cells in synovial uflid increase in the knee with degenerated cartilage and osteoarthritis. J. Orthop. Res. 30, 943-949 (2012).

    9. Zhang, S., Muneta, T., Morito, T., Mochizuki, T. & Sekiya, I. Autologous synovial uflid enhances migration of mesenchymal stem cells from synovium of osteoarthritis patients in tissue culture system. J. Orthop. Res. 26, 1413-1418 (2008).

    10. Aksu, A. E., Rubin, J. P., Dudas, J. R. & Marra, K. G. Role of gender and anatomical region on induction of osteogenic diefrentiation of human adipose-derived stem cells. Ann. Plast. Surg. 60, 306-322 (2008).

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