publication . Article . 2013


Mackenzie, N C W; Staines, K A; Zhu, D; Genever, P; MacRae, V E; Edinburgh; York;
Open Access English
  • Published: 01 Oct 2013 Journal: Cell Biochemistry and Function, volume 32, issue 2, pages 209-216 (issn: 0263-6484, eissn: 1099-0844, Copyright policy)
  • Publisher: Blackwell Publishing Ltd
  • Country: United Kingdom
Vascular calcification shares many similarities with skeletal mineralisation and involves the phenotypic trans-differentiation of vascular smooth muscle cells (VSMCs) to osteoblastic cells within a calcified environment. Various microRNAs (miRs) are known to regulate cell differentiation; however, their role in mediating VSMC calcification is not fully understood. miR-microarray analysis revealed the significant down-regulation of a range of miRs following nine days in culture, including miR-199b, miR-29a, miR-221, miR-222 and miR-31 (p<0.05). Subsequent studies investigated the specific role of the miR-221/222 family in VSMC calcification. Real-time quantitativ...
free text keywords: Research Articles, calcification, Enpp1, microarray, miRNA, vascular smooth muscle cell, Clinical Biochemistry, Cell Biology, Biochemistry, General Medicine, Biology, Transfection, Cell, medicine.anatomical_structure, medicine, RUNX2, microRNA, medicine.disease, Cellular differentiation, Real-time polymerase chain reaction, Vascular smooth muscle
45 references, page 1 of 3

1. Berezikov E, Guryev V, van de Belt J, Wienholds E, Plasterk RHA, Cuppen E. Phylogenetic shadowing and computational identification of human microRNA genes. Cell 2005; 120: 21-24.

2. Bentwich I, Avniel A, Karov Y, et al. Identification of hundreds of conserved and nonconserved human microRNAs. Nat Genet 2005; 37: 766-770. [OpenAIRE]

3. Cui RR, Li SJ, Liu LJ, et al. MicroRNA-204 regulates vascular smooth muscle cell calcification in vitro and in vivo. Cardiovasc Res 2012; 96: 320-329.

4. Li P, Liu Y, Yi B, et al. MicroRNA-638 is highly expressed in human vascular smooth muscle cells and inhibits PDGF-bb-induced cell proliferation and migration through targeting orphan nuclear receptor nor1. Cardiovasc Res 2013; 96: 320-329.

5. Leeper NJ, Raiesdana A, Kojima Y, et al. MicroRNA-26a is a novel regulator of vascular smooth muscle cell function. J Cell Physiol 2011; 226: 1035-1043. [OpenAIRE]

6. Gui T, Zhou GY, Sun YJ, et al. MicroRNAs that target ca2+ transporters are involved in vascular smooth muscle cell calcification. Lab Invest 2012; 92: 1250-1259.

7. Coleman CB, Lightell DJ, Jr., Moss SC, Bates M, Eugene Parrino P, Cooper Woods T. Elevation of miR-221 and 222 in the internal mammary arteries of diabetic subjects and normalization with metformin. Mol Cell Endocrinol 2013; 374(1-2): 125-9.

8. Johnson K, Polewski M, van Etten D, Terkeltaub R. Chondrogenesis mediated by PPi depletion promotes spontaneous aortic calcification in npp1 / mice. Arterioscler Thromb Vasc Biol 2005; 25: 686-691.

9. Johnson KA, Polewski M, Terkeltaub RA. Transglutaminase 2 is central to induction of the arterial calcification program by smooth muscle cells. Circ Res 2008; 102: 529-537.

10. Zhu D, Mackenzie NCW, Millan JL, Farquharson C, MacRae VE. The appearance and modulation of osteocyte marker expression during calcification of vascular smooth muscle cells. PLoS One 2011; 6: e19595.

11. Zhu D, Mackenzie NC, Millan JL, Farquharson C, Macrae VE. A protective role for FGF-23 in local defence against disrupted arterial wall integrity? Mol Cell Endocrinol 2013; 372: 1-11.

12. Newton PT, Staines KA, Spevak L, et al. Chondrogenic ATDC5 cells: An optimised model for rapid and physiological matrix mineralisation. Int J Mol Med 2012; 30: 1187-1193. [OpenAIRE]

13. Staines KA, Mackenzie NCW, Clarkin CE, et al. MEPE is a novel regulator of growth plate cartilage mineralization. Bone 2012; 51: 418-430. [OpenAIRE]

14. Farquharson C, Lester D, Seawright E, Jefferies D, Houston B. Microtubules are potential regulators of growth-plate chondrocyte differentiation and hypertrophy. Bone 1999; 25: 405-412.

15. Sun K, Wang W, Lei S-t, Wu C-t, Li G-x. MicroRNA-221 promotes colon carcinoma cell proliferation in vitro by inhibiting Cdkn1c/p57 expression. Journal of Southern Medical University 2011; 31: 1885-1889.

45 references, page 1 of 3
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publication . Article . 2013


Mackenzie, N C W; Staines, K A; Zhu, D; Genever, P; MacRae, V E; Edinburgh; York;