Commitment and potential of dental pulp stem cells: their role in directing dental tissue regeneration

Doctoral thesis English OPEN
Lee, Chi
  • Subject: RK
    mesheuropmc: stomatognathic diseases | stomatognathic system

Dental pulp stem cells (DPSCs) play an integral role during dentine injury where they migrate towards the injury site, proliferate, differentiate into odontoblast-like\ud cells and secrete a mineralised matrix, protecting the vital pulp tissue and preserving the tooth organ. Dentine matrix proteins (DMP) may have a role in stimulating this\ud reparative dentine formation. Multiple adult stem cells make up the DPSC population. Within this study progenitor\ud cells were isolated from dental pulp by preferential fibronectin adherence (FNA) or using magnetic activated cell sorting (MACS) to isolate P75 expressing neural crest\ud cells. Clonally expanded colonies were established. The proliferative FNA and P75-sorted populations were shown to be distinct progenitors with differing morphology\ud and stem cell marker expression. One FNA clonal population was differentiated into mesenchymal lineages that established it as a multipotent DPSC population.\ud Clonal populations were supplemented with DMP in vitro to examine the potential role of DMP in modulating cell behaviour during dentine injury. Supplementing with\ud DMP had a dose dependent response on DPSC viability, increased cell numbers,reduced apoptosis and promoted cell migration, suggesting that the growth factors in\ud DMP may have a positive synergistic effect on DPSC behaviour. Fluorescently stained DPSCs were microinjected into transverse tooth slices and placed in culture to develop an ex vivo model to study DPSC behaviour in a tooth\ud environment. Injected cells remained viable after 7 days of culture, providing proof of concept data that DPSCs can be localised for study in situ. This thesis was successful in the isolation of clonal populations representing\ud progenitor cells with different characteristics. Supplementing DPSCs with DMP promoted cell behaviour facilitating reparative dentinogenesis, indicating a potential clinical application for DMP in restorative therapy. The study of DPSCs using the ex\ud vivo model will be important in further development of these novel therapies for dental tissue regeneration.
  • References (150)
    150 references, page 1 of 15

    Downer, M. C. 1993. Changing trends in dental caries experience in Great Britain. Adv Dent Res 7(1), pp. 19-24.

    Dowthwaite, G. P. et al. 2004. The surface of articular cartilage contains a progenitor cell population. J Cell Sci 117(Pt 6), pp. 889-897.

    Driessens, F. C. M. and Woltgens, J. H. M. 1986. Tooth Development and Caries. Florida, USA: CRC Press Inc.

    Ducy, P. et al. 1996. Increased bone formation in osteocalcin-deficient mice. Nature 382(6590), pp. 448-452.

    Dung, S. Z. et al. 1995. Effect of lactic acid and proteolytic enzymes on the release of organic matrix components from human root dentin. Caries Res 29(6), pp. 483-489.

    Dupin, E. et al. 2007. Neural crest progenitors and stem cells. C R Biol 330(6-7), pp. 521-529.

    Dupin, E. et al. 2000. Endothelin 3 induces the reversion of melanocytes to glia through a neural crest-derived glial-melanocytic progenitor. Proc Natl Acad Sci U S A 97(14), pp. 7882-7887.

    Dupin, E. et al. 1998. The avian embryo as a model in developmental studies: chimeras and in vitro clonal analysis. Curr Top Dev Biol 36, pp. 1-35.

    Duque, C. et al. 2006. Reactionary dentinogenesis after applying restorative materials and bioactive dentin matrix molecules as liners in deep cavities prepared in nonhuman primate teeth. J Oral Rehabil 33(6), pp. 452-461.

    Echelard, Y. et al. 1994. Cis-acting regulatory sequences governing Wnt-1 expression in the developing mouse CNS. Development 120(8), pp. 2213-2224.

  • Metrics
    0
    views in OpenAIRE
    0
    views in local repository
    104
    downloads in local repository

    The information is available from the following content providers:

    From Number Of Views Number Of Downloads
    Online Research @ Cardiff - IRUS-UK 0 104
Share - Bookmark