publication . Article . Other literature type . 2012

The cytotoxicity of polycationic iron oxide nanoparticles: Common endpoint assays and alternative approaches for improved understanding of cellular response mechanism

Lijun Wang; Clare Hoskins; Alfred Cuschieri;
Open Access English
  • Published: 01 Apr 2012 Journal: JOURNAL OF NANOBIOTECHNOLOGY (issn: 1477-3155, Copyright policy)
  • Country: United Kingdom
<p>Abstract</p> <p>Background</p> <p>Iron oxide magnetic nanoparticles (MNP's) have an increasing number of biomedical applications. As such in vitro characterisation is essential to ensure the bio-safety of these particles. Little is known on the cellular interaction or effect on membrane integrity upon exposure to these MNPs. Here we synthesised Fe<sub>3</sub>O<sub>4 </sub>and surface coated with poly(ethylenimine) (PEI) and poly(ethylene glycol) (PEG) to achieve particles of varying surface positive charges and used them as model MNP's to evaluate the relative utility and limitations of cellular assays commonly applied for nanotoxicity assessment. An alternat...
Medical Subject Headings: technology, industry, and agriculturemacromolecular substances
free text keywords: Q1, R1, Research, Magnetic nanoparticle, Cellular interaction, Cell membrane, Cytotoxicity, Cell viability assay, Atomic force microscopy, Zeta potential, Biotechnology, TP248.13-248.65, Medical technology, R855-855.5, Molecular Medicine, Applied Microbiology and Biotechnology, Medicine (miscellaneous), Bioengineering, Pharmaceutical Science, Biomedical Engineering, Chemistry, Iron oxide nanoparticles, chemistry.chemical_compound, Particle size, Ethylene glycol, medicine.anatomical_structure, medicine, Cell morphology, Nanotoxicology, Membrane, Biophysics, Magnetic nanoparticles, Biochemistry
Funded by
RCUK| Magnetic nanoparticles for nerve regeneration
  • Funder: Research Council UK (RCUK)
  • Project Code: EP/H007040/1
  • Funding stream: EPSRC
RCUK| Biomagnetic retraction for endoscopic surgery
  • Funder: Research Council UK (RCUK)
  • Project Code: EP/H010033/1
  • Funding stream: EPSRC
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