Investigating the effects of an oral fructose challenge on hepatic ATP reserves in healthy volunteers: a 31P MRS study

Article English OPEN
Bawden, Stephen ; Stephenson, M.C. ; Ciampi, Elisabetta ; Hunter, K. ; Marciani, Luca ; MacDonald, Ian A. ; Aithal, Guruprasad P. ; Morris, P.G. ; Gowland, Penny A. (2015)

Background: Impaired homeostasis of hepatic ATP has been associated with NAFLD. An intravenous fructose infusion has been shown to be an effective challenge to monitor the depletion and subsequent recovery of hepatic ATP reserves using 31P MRS.\ud Aims: The purpose of this study was to evaluate the effects of an oral rather than intravenous fructose challenge on hepatic ATP reserves in healthy subjects.\ud Methods: Self-reported healthy males were recruited. Following an overnight fast, baseline liver glycogen and lipid levels were measured using Magnetic Resonance Spectroscopy (MRS). Immediately after consuming a 500 ml 75 g fructose drink (1275 kJ) subjects were scanned continuously for 90 min to acquire dynamic 31P MRS measurements of liver ATP reserves.\ud Results: A significant effect on ATP reserves was observed across the time course (P < 0.05). Mean ATP levels reached a minimum at 50 min which was markedly lower than baseline (80 ± 17% baseline, P < 0.05). Subsequently, mean values tended to rise but did not reach statistical significance above minimum. The time to minimum ATP levels across subjects was negatively correlated with BMI (R2 ¼ 0.74, P < 0.005). Rates of ATP recovery were not significantly correlated with BMI or liver fat levels, but were negatively correlated with baseline glycogen levels (R2 ¼ 0.7, P < 0.05).\ud Conclusions: Depletion of ATP reserves can be measured non-invasively following an oral fructose challenge using 31P.
  • References (6)

    Mansell, P.G. Morris, and I.A. Macdonald. Variability in fasting lipid and glycogen contents in hepatic and skeletal muscle tissue in subjects with and without type 2 diabetes: a 1H and 13C MRS study. NMR in Biomedicine, 2013, 26(1518 - 1526; Awad, S., M.C. Stephenson, E. Placidi, L. Marciani, D. Constantin-Teodosiu, P.A. Gowland, R.C. Spiller, K.C.H. Fearon, P.G. Morris, I.A. Macdonald, and D.N. Lobo. The effects of fasting and refeeding with a 'metabolic preconditioning' drink on substrate reserves and mononuclear cell mitochondrial function. Clinical Nutrition, 2010, 29(4), 538-544; Stefan, D., F. Di Cesare, A. Andrasescu, E. Popa, A. Lazariev, E. Vescovo, O. Strbak, S.

    Williams, Z. Starcuk, M. Cabanas, D. van Ormondt, and D. Graveron-Demilly. Quantitation of magnetic resonance spectroscopy signals: the jMRUI software package. Measurement Science & Technology, 2009, 20(10), Jovanovic, A., E. Leverton, B. Solanky, B. Ravikumar, J.E.M. Snaar, P.G. Morris, and R. Taylor.

    The second-meal phenomenon is associated with enhanced muscle glycogen storage in humans. Clinical Science, 2009, 117(3-4), 119-127; Korieh, A. and G. Crouzoulon. Dietary-Regulation of Fructose Metabolism in the Intestine and in the Liver of the Rat - Duration of the Effects of a High Fructose Diet after the Return to the Standard Diet. Archives Internationales De Physiologie De Biochimie Et De Biophysique, 1991, 99(6), 455-460; Ouyang, X., P. Cirillo, Y. Sautin, S. McCall, J.L. Bruchette, A.M. Diehl, R.J. Johnson, and M.F.

    Journal of Hepatology, 2008, 48(6), 993-999; Buemann, B., H. Gesmar, A. Astrup, and B. Quistorff. Effects of oral D-tagatose, a stereoisomer of D-fructose, on liver metabolism in man as examined by P-31-magnetic resonance spectroscopy. Metabolism-Clinical and Experimental, 2000, 49(10), 1335-1339; Kwiatek, M.A., D. Menne, A. Steingoetter, O. Goetze, Z. Forras-Kaufman, E. Kaufman, H.

    Fruehauf, P. Boesiger, M. Fried, W. Schwizer, and M.R. Fox. Effect of meal volume and calorie load on postprandial gastric function and emptying: studies under physiological conditions by combined fiber-optic pressure measurement and MRI. American Journal of Physiology-Gastrointestinal and Liver Physiology, 2009, 297(5), G894-G901; Rumessen, J.J. and E. Gudmandhoyer. Absorption Capacity of Fructose in Healthy-Adults - Comparison with Sucrose and Its Constituent Monosaccharides. Gut, 1986, 27(10), 1161- 1168; Szendroedi, J., M. Chmelik, A.I. Schmid, P. Nowotny, A. Brehm, M. Krssak, E. Moser, and M.

    Roden. Abnormal Hepatic Energy Homeostasis in Type 2 Diabetes. Hepatology, 2009, 50(4), 1079-1086; Bollen, M., S. Keppens, and W. Stalmans. Specific features of glycogen metabolism in the liver. Biochemical Journal, 1998, 336(19-31; Lim, E.L., K.G. Hollingsworth, F.E. Smith, P.E. Thelwall, and R. Taylor. Effects of raising muscle glycogen synthesis rate on skeletal muscle ATP turnover rate in type 2 diabetes. American Journal of Physiology-Endocrinology and Metabolism, 2011, 301(6), E1155-E1162; Gallis, J.L., H. Gin, H. Roumes, and M.C. Beauvieux. A metabolic link between mitochondrial ATP synthesis and liver glycogen metabolism: NMR study in rats re-fed with butyrate and/or glucose. Nutrition & Metabolism, 2011, 8( Choi, Y.K., F.C. Johlin, R.W. Summers, M. Jackson, and S.S.C. Rao. Fructose intolerance: An under-recognized problem. American Journal of Gastroenterology, 2003, 98(6), 1348-1353;

  • Metrics
    No metrics available
Share - Bookmark