A self-assembling fluorescent dipeptide conjugate for cell labelling
Hamley, Ian W.
Smith, Andrew M.
Gouveia, Ricardo M.
Connon, Che J.
- Publisher: Elsevier
Colloids and Surfaces B: Biointerfaces
(issn: 0927-7765, vol:
218 Environmental engineering | DELIVERY | PEPTIDE | Biotechnology | Surfaces and Interfaces | 114 Physical sciences | Peptides | SUPRAMOLECULAR HYDROGELS | Self-assembly | DERIVATIVES | 221 Nanotechnology | NANOPARTICLES | Physical and Theoretical Chemistry | Fluorescence | 214 Mechanical engineering | Colloid and Surface Chemistry | NANOFIBERS/HYDROGELS | Peptide conjugates | ENZYMATIC FORMATION | SMALL MOLECULES | CYTOTOXICITY | ENHANCE
Derivatives of fluorophore FITC (fluorescein isothiocyanate) are widely used in bioassays to label proteins and cells. An N-terminal leucine dipeptide is attached to FITC, and we show that this simple conjugate molecule is cytocompatible and is uptaken by cells (human dermal and corneal fibroblasts) in contrast to FITC itself. Co-localisation shows that FITC-LL segregates in pen-nuclear and intracellular vesicle regions. Above a critical aggregation concentration, the conjugate is shown to self-assemble into beta-sheet nanostructures comprising molecular bilayers. (C) 2015 The Authors. Published by Elsevier B.V. This is an open access article under the CC BY license (http://creativecommons.orgflicenses/by/4.0/).