Continuous Flow One Chamber Chemostat: Effect of Selected Antimicrobials on the Human Intestinal Microflora

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Carman, Robert J. ; Woodburn, Mary Alice (2011)
  • Publisher: Microbial Ecology in Health and Disease
  • Journal: Microbial Ecology in Health and Disease (issn: 1651-2235)
  • Related identifiers: doi: 10.3402/mehd.v12i1.8049

Low levels of antibiotics in the human food chain are a public health concern. Current FDA-CVM guidelines state that the maximum amount of microbiologically active residue that may be consumed by a person in one day for life is 1.5 mg. This is called the Acceptable Daily Intake or ADI. The guidelines also suggest additional but unspecified testing of any compound for which an ADI greater than 1.5 mg/person/day is sought. We tested tetracycline (0, 0.15, 1.5 and 15 µg/mL), neomycin (0, 1.78, 17.8 and 178 µg/mL), erythromycin (0, 1.5, 15 and 150 µg/mL) and ciprooxacin at (0, 0.43, 43, and 43 µg/mL). Based on published data of the fecal levels resulting from standard oral doses, these levels correspond to 0, 1.5, 15, 150 mg/person/d for tetracycline, neomycin and erythromycin, and 0, 0.48, 4.8 and 48 mg/person/d for ciprofloxacin. For ease of reference however, we will refer to these various challenge levels as the no-drug control and the low, medium and high doses.
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