Restriction Endonuclease DNA Analysis of Bacteroides fragilis

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Namavar, F. ; Van Steenbergen, T. J. M. ; Verweij-Van Vught, A. M. J. J. ; Maclaren, D. M. (2011)
  • Publisher: Microbial Ecology in Health and Disease
  • Journal: Microbial Ecology in Health and Disease (issn: 1651-2235)
  • Related identifiers: doi: 10.3402/mehd.v4i2.7616
  • Subject:
    mesheuropmc: food and beverages

Hind III and Bam HI restriction endonuclease digests of genomic DNA of 25 Bacteroides fragilis strains, isolated from faeces, intestinal mucosa and infections were investigated. DNA analysis of single isolates from 10 patients with a B. fragilis infection revealed 10 different DNA profiles. DNA isolated from all 15 strains from faeces and intestinal mucosa of one patient showed identical patterns, confirming occurrence of one dominant strain in an individual. Restriction endonuclease DNA analysis provides a sensitive and useful technique for the typing of B. fragilis in epidemiological studies.Keywords: Bacteroides fragilis; Epidemiology; Restriction endonuclease.
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