Probiotic strains of Lactobacillus and Bifidobacterium affect the translocation and intestinal load of Enterobacteriaceae differently after D-galactosamine-induced liver injury in rats

Article English OPEN
Osman, Nadia ; Adawi, Diya ; Ahrne, Siv ; Jeppsson, Bengt ; Molin, Göran (2011)
  • Publisher: Microbial Ecology in Health and Disease
  • Journal: Microbial Ecology in Health and Disease (issn: 1651-2235, eissn: 1651-2235)
  • Related identifiers: doi: 10.3402/mehd.v17i1.7808
  • Subject:
    mesheuropmc: food and beverages

In an acute liver injury model we compared the effects of different Lactobacillus and Bifidobacterium strains on bacterial translocation, intestinal load of Enterobacteriaceae and the extent of liver injury. This was an experimental study carried out in an university hospital in Sweden. Sprague-Dawley rats were divided into six groups: liver injury control and five groups of liver injury with administration of three different Lactobacillus and two Bifidobacterium strains (orally twice daily for 8 days). Liver injury was induced on the eighth day by intraperitoneal injection of D-galactosamine (1.1 g/kg body weight). The main outcome measures were samples collected 24 h after injury. Liver enzymes and bilirubin serum levels, bacterial translocation (to arterial and portal blood, liver and mesenteric lymph nodes), intestinal load of Enterobacteriaceae in relation to lactobacilli and the total bacterial load were evaluated and randomly amplified polymorphic DNA (RAPD) analysis of translocating bacteria was carried out. Lactobacillus plantarum DSM 9843, Lactobacillus gasseri 5B3 and Bifidobacterium infantis DSM 15158 decreased bacterial translocation to the liver compared with the liver injury control group. Lactobacillus paracasei DSM 13434 translocated to the liver. The Enterobacteriaceae count in the caecum decreased in the L. plantarum DSM 9843, L. gasseri 5B3, Bifidobacterium ‘urinalis’ 3B1 and B. infantis DSM 15158 groups, while all the administered probiotics decreased it in the colon. The levels of alanine aminotransferase (ALT) and bilirubin were significantly lower in the L. plantarum DSM 9843 and B. infantis DSM 15158 groups compared with the liver injury control group. All test strains except L. paracasei DSM 13434 inhibited translocation to the liver. Instead, L. paracasei was found in the liver and it also failed to decrease the load of Enterobacteriaceae in caecum. The best strains in protecting the liver during injury were L. plantarum DSM 9843 and B. infantis DSM 15158, as reflected by bilirubin and liver enzymes. Thus, there are major effectual differences between strains/species. In contrast, the phylogenetically most diverse strains, L. plantarum DSM 9843 and B. infantis DSM 15158, exercised the same effects.Key words: Bacterial translocation, D-galactosamine, liver injury, probiotics, rats
  • References (47)
    47 references, page 1 of 5

    1. Saadia R, Schein M, MacFarlane C, Boffard KD. Gut barrier function and the surgeon. Br J Surg. 1990;77:487 /92.

    2. Deitch EA. Multiple organ failure: pathophysiology and potential future therapy. Ann Surg. 1992;216:117 /34.

    3. Ahrendt GT, Barbul A. Nutrition and multiple organ dysfunction syndrome. Curr Opin Gastroenterol. 1994; 10:203 /9.

    4. Deitch EA: Nutrition and the gut mucosal barrier. Curr Opin Gen Surg 1993;85 /91.

    5. Helton WS. Nutritional issues in hepatobiliary surgery. Semin Liver Dis. 1994;14:140 /57.

    6. Kasravi FB, Wang X, Guo W, Andersson R, Norgren L, Jeppsson B, et al. Reticuloendothelial system function in acute liver injury induced by D-galactosamine. J Hepatol. 1995; 23:727 /33.

    7. Adachi Y, Moore LE, Bradford BU, Gao W, Thurman RG. Antibiotics prevent liver injury in rats following long-term exposure to ethanol. Gastroenterology. 1995;108:218 /24.

    8. Nanji AA, Khettry U, Sadrzadeh SMH. Lactobacillus feeding reduces endotoxemia and severity of experimental alcoholic liver disease. Proc Soc Exp Biol Med. 1994;205:243 /7.

    9. Nolan JP. Endotoxin, reticuloendothelial function, and liver injury. Hepatology. 1981;1:458 /65.

    10. Teitelbaum JE, Walker WA. Nutritional impact of pre- and probiotics as protective gastrointestinal organisms. Annu Rev Nutr. 2002;22:107 /38.

  • Metrics
    No metrics available
Share - Bookmark