Colonisation of Germ-free Mice by Segmented Filamentous Bacteria after Oral Administration of Various Murine Intestinal Wall Preparations
Klaasen, H. L. B. M.
Koopman, J. P.
Van Den Brink, M. E.
Van Wezel, H. P. N.
Scholten, P. M.
Beynen, A. C.
- Publisher: Microbial Ecology in Health and Disease
Microbial Ecology in Health and Disease
(issn: 1651-2235, eissn: 1651-2235)
Intestinal suspensions containing segmented filamentous bacteria (SFBs) were given orally to germ-free mice in an attempt to pave the way for the production of mice mono-associated with SFBs. The effects of dilution, sonication and chloroform/ethanol treatment of intestinal suspensions on SFB colonisation were investigated. Colonisation density of SFBs in the germ-free mice was dependent on the number of SFBs in the administered suspension. Sonication of suspensions had no effect. Chloroform/ethanol treatment of suspensions resulted in the production of di-associated mice, containing both SFBs and a non-characterised clostridium in their small intestine. It is suggested that, through specific elimination of the clostridium, these di-associated animals may be used to produce mice containing SFBs alone.Keywords: Colonisation; Intestinal bacteria; Mouse; Segmented filamentous bacteria.