Influence of Clostridium botulinum Type A Toxic Culture Supernatant on Intestinal Motility and the Ileo-caecal Junction in the Infant Mouse

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Conway, P. L. ; Adams, R. F. (2011)
  • Publisher: Microbial Ecology in Health and Disease
  • Journal: Microbial Ecology in Health and Disease (issn: 1651-2235, eissn: 1651-2235)
  • Related identifiers: doi: 10.3402/mehd.v1i4.7423
  • Subject:
    mesheuropmc: digestive system | digestive, oral, and skin physiology

A diluted toxic culture supernatant of a Clostridium botulinum Type A was inoculated intragastrically into 10 d old mice and the effect on gut motility was quantified. Transit time of a marker through the gut was increased from 3.5 h in controls to at least 6 h in the presence of the toxic supernatant. The ileo-caecal junction was open in all mice treated with the toxic supernatant but was closed in control mice. In treated mice there was significant shedding of enterocytes from the ileal villi. The effect of the supernatant on transit time was neutralised when the supernatant was treated with antiserum to Type A toxin before inoculation. It was concluded that the changes in the gut were probably effected by Type A toxin in the supernatant.Keywords: Intestinal motility; Botulinum toxin; Constipation.
  • References (15)
    15 references, page 1 of 2

    1. Adams RF, Conway PL. (1981). The effect of erythrosine on the surface-associated bacteria of the rat stomach and caecum. Journal of Applied Bacteriology 51, 171-181.

    2. Arnon SS, Midura TF, Clay SA, Wood RM, Chin J. (1 977). Infant botulism. Journal of the American Medical Association 237, 1946-1951 .

    3. Feldman RA. (1979). Introduction to seminar on infant botulism. Review of Infectious Diseases 1, 612-6 13.

    4. Johnson RO, Clay SA, Arnon S. (1979). Diagnosis and management of infant botulism. American Journal of Diseases in children 133,586-593.

    5 . McDonel JL, Duncan CL. (1975). Effects ofclostridium perfringens enterotoxin on metabolic indexes of everted rat ileal sacs. Infection and Immunity 12, 274-280.

    6. Mery F. (1947). Le botulisme chez le chien. Bullitin Academy of Vitirinaires,France 20,28-32.

    7. Moberg LJ, Sugiyama N. (1979). Microbial ecological basis of infant botulism as studied with germfree mice. Infection and Immunity 25,653-657.

    8. Murrell WG, Ouvrier RA, Stewart BJ, Dorman DC. (1981). Infant botulism in a breast fed infant from rural New South Wales. Medical Journal of Australia 1,583-585.

    9. Ohishi I, Odagiri Y. (1984). Histopathological effect of botulinum C2 toxin on mouse intestines. Infection and Immunity 43,5458.

    10. Pickett J, Berg B, Chaplin E, Brunstetter-Shafer M. (1976). Syndrome of botulism in infancy: clinical and electrophysiologic study. New England Journal of Medicine 295,770-772.

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