Colonization Resistance Induced in Mice Orogastrically Dosed with Human Faecal Homogenates from Different Donors

Article English OPEN
Henriksson, A. ; Conway, P. L. (2011)
  • Publisher: Microbial Ecology in Health and Disease
  • Journal: Microbial Ecology in Health and Disease (issn: 1651-2235)
  • Related identifiers: doi: 10.3402/mehd.v13i2.8010
  • Subject:
    mesheuropmc: digestive, oral, and skin physiology

Human faecal samples from various donors were assessed for the capacity to provide protection against Salmonella typhimurium both in vitro and in vivo. Faecal material was collected from four healthy human subjects. Populations of anaerobic bacteria and enterics were enumerated using selective media and the isolates screened for the capacity to inhibit the in vitro growth of S. typhimurium. Isolates with inhibitory activity against S. typhimurium were detected in two out of four subjects. These isolates accounted for 8 and 2% of the total culturable faecal population, which corresponded to 6 x 108 and 2 x 108 CFU per g, respectively. Ex-germ-free mice (Balb/C) were dosed with faecal material and then challenged with S. typhimurium. Protection against infection was monitored by enumerating the faecal levels of the pathogen. Human flora associated mice had high faecal levels of anaerobes (1010 CFU per g). Faecal levels of enterics in these animals ranged from 104 to 108 CFU per g. Animals dosed with human faecal material gained protection against gastrointestinal colonisation by S. typhimurium. In these animals, the faecal levels of Salmonella ranged from 1 x 102 to 5 x 105 CFU per g, compared with the levels of greater than 5 x 106 CFU per g in animals not previously associated with the human flora. Human faecal material containing the highest levels of inhibitory isolates provided the best protection in mice, against colonisation by S. typhimurium.Keywords: colonization resistance, germ-free.
  • References (14)
    14 references, page 1 of 2

    1. van der Vaaij D, Berghuis-deVries JM, Lekkerkerk van der Wees JEC. Colonization resistance of the digestive tract in conventional and antibiotic-treated mice. J. Hyg. 1971; 69: 405 - 11.

    2. Wells CL, Maddaus MA, Jechorek RP, Simmons RL. Role of intestinal anaerobic bacteria in colonization resistance. Eur. J. Clin. Microbiol. Infect. Dis. 1988; 7: 107 -13.

    3. Voravuthikunchai SP, Lee A. Cecectomy causes long-term reduction of colonization resistance in the mouse gastrointestinal tract. Infect. Immun. 1987; 55: 995 - 9.

    4. Blomberg L, Conway PL. An in vitro study of colonisation resistance to Escherichia coli strain Bd 1107:7508 (K88) in relation to indigenous squamous gastric colonisation in piglets of varying ages. Microb. Ecol. Health Dis. 1989; 2: 285 - 91.

    5. Maejima K, Tajima Y. Association of gnotobiotic mice with various organisms isolated from conventional mice. Jpn. J. Exp. Med. 1973; 43: 289 - 96.

    6. Berg RD, Owens WE. Inhibition of translocation of viable Escherichia coli from the gastrointestinal tract to the mesenteric lymph nodes and other organs in a gnotobiotic mouse model. Infect. Immun. 1979; 25: 820 - 7.

    7. Berg RD. Inhibition of Escherichia coli translocation from the gastrointestinal tract by normal cecal ora in gnotobiotic or antibiotic-decontaminated mice. Infect. Immun. 1980; 29: 1073 - 81.

    8. Freter R, Abrams GD. Functions of various intestinal bacteria in converting germ free mice to the normal state. Infect. Immun. 1972; 6: 119 - 26.

    9. Hudault S, Lievin V, Bernetcamamrd MF, Servin AL. Antagonistic activity exerted in vivo and in vitro by Lactobacillus casei (strain GG) against Salmonella typhimurium C5 infection. Appl. Environ. Microbiol. 1997; 63: 513 - 8.

    10. Bernet-Camard MF, Lievin V, Bassart D, Neeser JR, Servin AL, Hudault S. The human Lactobacillus acidophilus strain LA1 secrets a nonbacteriocin antibacterial substance(s) active in vitro and in vivo. Appl. Environ. Microbiol. 1997; 63: 2747 - 53.

  • Metrics
    No metrics available
Share - Bookmark