Preliminary Studies on Attachment of the ‘Bacteroides fragilis group’ to Porcine Intestinal Brush Borders

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Haugstad, May Britt ; Næss, Vigfrid ; Hofstad, T. (2011)
  • Publisher: Microbial Ecology in Health and Disease
  • Journal: Microbial Ecology in Health and Disease (issn: 1651-2235, eissn: 1651-2235)
  • Related identifiers: doi: 10.3402/mehd.v2i3.7465
  • Subject:
    mesheuropmc: food and beverages

Adhesion was studied by phase contrast microscopy, scanning (SEM), and transmission (TEM) electron microscopy. Twenty-three of 26 Bacteroides fragilis strains adhered to the isolated brush border membranes. The percentage of bacterial cells that adhered varied between strains from 10 to 38. Seven of 25 strains belonging to the other species within the ‘Bacteroides fragilis group’ contained cells that adhered. The attached bacteria showed no outer appendages or outer ruthenium red positive material when examined by SEM and TEM.Keywords: Bacteroides fragilis; Intestinal brush border membranes; Adhesion.
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