Detection of biomarkers using recombinant antibodies coupled to nanostructured platforms

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Kierny, Michael R. ; Cunningham, Thomas D. ; Kay, Brian K. (2012)
  • Publisher: Co-Action Publishing
  • Journal: Nano Reviews, volume 3 (eissn: 2000-5121)
  • Related identifiers: pmc: PMC3404449, doi: 10.3402/nr.v3i0.17240, doi: 10.3402/nano.v3i0.17240
  • Subject: phage-display | Review Article | carbon nanotube | scFv | Fab | affinity maturation | phage-display; scFv; Fab; therapeutic antibody; affinity maturation; mutagenesis nanotechnology; carbon nanotube; nanoshell; electrochemical detection | therapeutic antibody | nanotechnology | electrochemical detection | nanoshell | mutagenesis

The utility of biomarker detection in tomorrow’s personalized health care field will mean early and accurate diagnosis of many types of human physiological conditions and diseases. In the search for biomarkers, recombinant affinity reagents can be generated to candidate proteins or post-translational modifications that differ qualitatively or quantitatively between normal and diseased tissues. The use of display technologies, such as phagedisplay, allows for manageable selection and optimization of affinity reagents for use in biomarker detection. Here we review the use of recombinant antibody fragments, such as scFvs and Fabs, which can be affinity-selected from phagedisplay libraries, to bind with both high specificity and affinity to biomarkers of cancer, such as Human Epidermal growth factor Receptor 2 (HER2) and Carcinoembryonic antigen (CEA). We discuss how these recombinant antibodies can be fabricated into nanostructures, such as carbon nanotubes, nanowires, and quantum dots, for the purpose of enhancing detection of biomarkers at low concentrations (pg/mL) within complex mixtures such as serum or tissue extracts. Other sensing technologies, which take advantage of ‘Surface Enhanced Raman Scattering’ (gold nanoshells), frequency changes in piezoelectric crystals (quartz crystal microbalance), or electrical current generation and sensing during electrochemical reactions (electrochemical detection), can effectively provide multiplexed platforms for detection of cancer and injury biomarkers. Such devices may soon replace the traditional time consuming ELISAs and Western blots, and deliver rapid, point-of-care diagnostics to market.Keywords: phage-display; scFv; Fab; therapeutic antibody; affinity maturation; mutagenesis; nanotechnology; carbon nanotube; nanoshell; electrochemical detection(Published: 23 July 2012)Citation: Nano Reviews 2012, 3: 17240 -
  • References (174)
    174 references, page 1 of 18

    1. Blonder J, Issaq HJ, Veenstra TD. Proteomic biomarker discovery: its more than just mass spectrometry. Electrophoresis 2011; 32: 1541 8. Available from: http://onlinelibrary.

    2. Simpson KL, Whetton AD, Dive C. Quantitative mass spectrometry-based techniques for clinical use: biomarker identification and quantification. J Chromatogr B Analyt Technol Biomed Life Sci 2009; 877: 1240 9. Available from: 8453

    3. Hunt DF, Yates JR III, Shabanowitz J, Winston S, Hauer CR. Protein sequencing by tandem mass spectrometry. Proc Natl Acad Sci USA 1986; 83: 6233 7. Available from: http://

    4. Rifai N, Gillette MA, Carr SA. Protein biomarker discovery and validation: the long and uncertain path to clinical utility. Nat Biotechnol 2006; 24: 971 83. Available from: http://

    5. Braunstein GD, Rasor J, Danzer H, Adler D, Wade ME. Serum human chorionic gonadotropin levels throughout normal pregnancy. Am J Obstet Gynecol 1976; 126: 678 81. Available from:

    6. Waller DK, Lustig LS, Smith AH, Hook EB. Alphafetoprotein: a biomarker for pregnancy outcome. Epidemiology 1993; 4: 471 6. Available from: epidem/Abstract/1993/09000/Alpha_Fetoprotein__A_Biomarker_ for_Pregnancy.14.aspx

    7. Thygesen K, Alpert JS, White HD. Universal definition of myocardial infarction. J Am Coll Cardiol 2007; 50: 2173 95. Available from: pii/S0735109707029579

    8. Pineda JA, Lewis SB, Valadka AB, Papa L, Hannay HJ, Heaton SC, et al. Clinical significance of alphaIIspectrin breakdown products in cerebrospinal fluid after severe traumatic brain injury. J Neurotrauma 2007; 24: 354 66. Available from: 10.1089/neu.2006.003789

    9. Banyan Biomarker Immunoassays. Available from: http:// [cited 15 December 2011].

    10. Gold P, Freedman SO. Demonstration of tumor-specific antigens in human colonic carcinomata by immunological tolerance and absorption techniques. J Exp Med 1965; 121: 439 62. Available from: 439.abstract

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