Adhesion of Streptococcus mutans to Saliva-coated Hydroxyapatite formed in situ in Microtitre Plates

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Laar, J. H. Van ; Soet, J. J. De ; Hogeveen, R. ; Graaff, J. De (2011)
  • Publisher: Microbial Ecology in Health and Disease
  • Journal: Microbial Ecology in Health and Disease (issn: 1651-2235, eissn: 1651-2235)
  • Related identifiers: doi: 10.3402/mehd.v9i1.8351

Adhesion of Streptococcus mutans to the salivary pellicle was studied in vitro by using microtitre plates coated in situ with hydroxyapatite (HA). Adhesion of S. mutans to these plates did not exhibit saturation kinetics and there was an almost linear relationship between the number of adhering cells and the total input of cells. This is in accordance with results obtained by scanning electron microscopy. S. mutans adhered not only to the salivary pellicle but also to bound S. mutans cells. This phenomenon, referred to as homologous coadhesion, resulted in the formation of aggregates of S. mutans cells. Homologous coadhesion of S. mutans was enhanced significantly by saliva. The presence of bound bacterial cells of Streptococcus gordonii strain HG222 or Streptococcus sanguis strain Bart 10 on saliva-coated HA increased the number of adhering S. mutans cells; the increase in the number of adhering S. mutans cells was most pronounced when the plates were precoated with the homologous S. mutans strain HG982. Other oral streptococci, when bound to saliva-coated HA, decreased adhesion of S. mutans (Streptococcus oralis strains NY583 and ST26, Streptococcus mitis strain HG168) or had no effect on adhesion (S. sanguis strain Bart4, S. gordonii strain MCC272, S. mitis strain ST3I). In conclusion, a new mechanism for accumulation of S. mutans on a saliva-coated HA surface has been proposed: the formation of aggregates of S. mutans cells.Keywords: Streptococcus mutans, adhesion, hydroxyapatite, microtitre plates, salivary pellicle.
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