The Presence of a Cytochrome P450-like Protein in the Human Intestinal Micro┬Ć ora Eubacterium aerofaciens

Article English OPEN
John, Gilbert H. ; Walls, Shannon ; Keith, Ronald ; Goodfox-Jones, Jodi ; Tucker, Kayleen ; Abraham, K. J. (2011)
  • Publisher: Microbial Ecology in Health and Disease
  • Journal: Microbial Ecology in Health and Disease (issn: 1651-2235, eissn: 1651-2235)
  • Related identifiers: doi: 10.3402/mehd.v13i1.7991

Eighteen human intestinal microbial strains were tested for the presence of cytochrome P450, an enzyme involved in detoxifying and toxifying various xenobiotics. The polymerase chain reaction (PCR) technique was used to amplify conserved regions within the gene. From the human intestinal micro ora Eubacterium aerofaciens, a DNA fragment of the expected size could be ampli. ed, for which a percentage homology of 20% was determined. Southern blot analysis, using Pseudomonas putida P450 CAM probes, showed reactions with probes at 45 and 55°C. The carbon monoxide (CO) difference spectrum produced a P450-like pro. le for E. aerofaciens, similar to P. putida P450 CAM. The presence of cytochrome P450 in human intestinal  ora may in uence the expression of hepatic cytochrome P450s.Keywords : human intestinal  ora, cytochrome P450, PCR, CO difference spectrum.
  • References (38)
    38 references, page 1 of 4

    1. McDanell RE, McLean AEM, Hanley AB, Heaney RK, Fenwick GR. Chemical and biological properties of indole glucosinolates (glucobrassicins): a review. Food Chem Toxicol 1988; 26: 59 - 70.

    2. Nugon-Brudon L, Rabot S. Glucosinolates and glucosinolate derivatives: implications for protection protecting against chemical carcinogenesis. Nutr Res Rev 1994; 7: 205 - 31.

    3. Duncan AJ, Milne JA. Glucosinolates. Aspects Appl Biol 1989; 19: 75 - 92.

    4. Nugon-Baudon L, Szylit O, Raibaud P. Production of toxic glucosinolate derivatives from rapeseed meal by intestinal micro ora of rat and chicken. J Sci Food Agric 1988; 43: 299 - 308.

    5. Nugon-Baudon L, Rabot S, Szylit O, Raibaud P. Glucosinolates toxicity in growing rats: interactions with the hepatic detoxi cation system. Xenobiotica 1990; 20: 223 - 30.

    6. Rabot S, Nugon-Baudon L, Szylit O. Alterations of the hepatic xenobiotic-metabolizing enzymes by a glucosinolaterich diet in germ-free rats: in uence of a pre-induction with phenobarbital. Br J Nutr 1993; 70: 347 - 54.

    7. Nathalie R, Nugon-Baudon L, Flinois J-P, Beaune P. Hepatic and intestinal cytochrome P-450, glutathione-S transferase and UDP-glucuronosyl transferase are affected by six types of dietary ber in rats inoculated with human whole fecal ora. J Nutr 1994; 124: 1581 - 7.

    8. Nugon-Baudon L, Robot S, Flinois JP, Lory S, Beaune Ph. Effects of the bacterial status of rats on the changes in some liver cytochrome P450 (EC apoprotein consequent to a glucosinolate-rich diet. Br J Nutr 1998; 80: 231 - 4.

    9. Caldwell DR, Bryant MP. Medium without rumen uid for nonselective enumeration and isolation of rumen bacteria. Appl Microbiol 1966; 14: 794 - 801.

    10. Sambrook J, Fritsch EF, Maniatis T. Molecular Cloning. The Laboratory Manual, 2nd Edition. Cold Spring Harbor Laboratory Press, 1989.

  • Metrics
    No metrics available
Share - Bookmark