publication . Article . 1991

Ultrastructural Histopathology of Vervet Monkey Colonic Epithelium After In Vitro Exposure to Cell-free Supernatants of Shigella Cultures

R. R. Hill; N. E. Collins; H. M. Cowley;
Open Access English
  • Published: 04 Sep 1991 Journal: Microbial Ecology in Health and Disease (issn: 1651-2235, eissn: 1651-2235, Copyright policy)
  • Publisher: Microbial Ecology in Health and Disease
Abstract
The full dysentery syndrome of human shigellosis is often preceded by a transient diarrhoea that may be induced by bacterial extracellular products before invasion of the colonic mucosa and development of subsequent pathology. To examine this hypothesis, we studied the effects of cell-free cultures of Shigella sp. on the ultrastructure of monkey colonic epithelium in vitro . Clinical isolates of shigella strains were grown in a niche-simulating medium. Sheets of colon wall collected from vervet monkeys were mounted between chambers in plastic blocks and bathed on the serosal side with a flow of minimal essential medium. Clarified 4 d culture supernatants of shig...
Subjects
free text keywords: Vervet monkey, biology.organism_classification, biology, Extracellular, Microbiology, Dysentery, medicine.disease, medicine, Shigella, medicine.disease_cause, Organ culture, Shigellosis, Epithelial Damage, Ultrastructure

1. Anand BS, Malhotra V, BhattacharyaSK, Datta P, Datta D, BhattacharyaNK, MukherjeePP,Pal SC. (1986). Rectal histology in acute bacillary dysentery. Gastroenterology 90,654660.

2. Cowley HM, Hill RH. (1985). Intestinal spirochaetosis of the vervet monkey. Onderstepoort Journal of Veterinary Research 52,47-50.

3. Donnellan W L .(1965).The structure of the colonic rnucosa. The epithelium and subepithelial reticulohistiocyticcomplex. Gastroenterology 49,49&5 14.

4. Fontaine A, Arondel J, Sansonetti PJ. (1988). Role of Shiga toxin in the pathogenesis of bacillary dysentery studied by using a tox-mutant of Shigella dysenteriae. Infection and Immunity 56,3099-3 109. [OpenAIRE]

5 . Hale TL, Formal SB. (1987). Pathogenesis of Shigella infections. Pathological and Zmrnunopathological Research 6,117-127.

6 . LaBrec EH, Schneider H, Magnani TJ, Formal SB. (1964). Epithelial cell penetration as an essentialstep in the pathogenesis of bacillary dysentery.Journal of Bacteriology 88,1503-1518. [OpenAIRE]

7. Mathan MM, Mathan VI. (1986). Ultrastructural pathology of the rectal mucosa in Shigella dysentery. American Journal of Pathology 123,2>38. [OpenAIRE]

8. O'Brien AD, Gentry MK, Thompson MR, Doctor BP, Gernski P, Formal SB. (1979). Shigellosis and Escherichia coli diarrhea: relative importance of invasive and toxigenic mechanisms. American Journal of Clinical Nutrition 32,229-233. [OpenAIRE]

9. Osada Y, Ogawa H. (1977). Phagocytosis stimulation by an extracellular product of virulent ShigellaJexneri 2a. Microbiology and Immunology 21,49-55.

10. Stephen J , Osborne MP. (1988). Pathophysiological mechanisms in diarrhoea1disease. In: Donachie W, Griffiths E, Stephen J (eds) Bacterial Infections of Respiratory and Gastrointestinal Mucosae. IRL, Oxford, pp 149-170.

11. Takeuchi Al, Sprinz H, Labrec EH, Formal SB. (1965). Experimental bacillary dysentery. An electron microscope study of the response of the intestinal mucosa to bacterial invasion. American Journal OfPathology 47,101 1-1020. [OpenAIRE]

12. Takeuchi A, Formal SB, Sprinz H. (1968). Experimental acute colitis in the rhesus monkey following peroral infection with Shigellafiexneri: an electronmicroscope study. American Journal of Pathology 52,503-527. [OpenAIRE]

Powered by OpenAIRE Open Research Graph
Any information missing or wrong?Report an Issue
publication . Article . 1991

Ultrastructural Histopathology of Vervet Monkey Colonic Epithelium After In Vitro Exposure to Cell-free Supernatants of Shigella Cultures

R. R. Hill; N. E. Collins; H. M. Cowley;