Comparison of Composition of Faecal Microbiota and Metabolism of Faecal Bacteria among 'Human-Flora-Associated' Mice Inoculated with Faeces from Six Different Human Donors

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Hirayama, K. ; Itoh, K. ; Takahashi, E. ; Mitsuoka, T. (2011)
  • Publisher: Microbial Ecology in Health and Disease
  • Journal: Microbial Ecology in Health and Disease (issn: 1651-2235, eissn: 1651-2235)
  • Related identifiers: doi: 10.3402/mehd.v8i5.8286
  • Subject:
    mesheuropmc: genetic structures | behavioral disciplines and activities | fluids and secretions | mental disorders

The composition of faecal microbiota, enzyme activities of faecal bacteria and concentrations of faecal putrefactive products and short chain fatty acids (SCFAs) were studied in 'human-flora-associated' (HFA) mice inoculated with six different human faeces. The composition of major faecal bacteria of HFA mice was similar to that of inoculated human faeces, although bifidobacteria were eliminated from some HFA mouse groups. β-Glucuronidase activity of intestinal bacteria in HFA mice was similar to that in humans. However nitroreductase activity was closer to that in conventional mice than in humans. Although the concentrations of faecal putrefactive products and SCFAs in HFA mice were much lower than those in humans, the composition of faecal SCFAs of HFA mice was similar to that of humans. Individual variations of putrefactive products and SCFAs observed among inoculated human faeces were not reflected in HFA mice, and variations among six HFA mice groups were not obvious. Bacterial composition and metabolism in HFA mice were maintained in their offspring by breeding. These findings indicate that HFA mice provide a stable and valuable tool for studying the ecosystem and metabolism of the human faecal microbiota, but they have some limitations as a model.Keywords: human-flora-associated mice, faecal microbiota, enzyme activities, putrefactive products, short chain. fatty acids
  • References (23)
    23 references, page 1 of 3

    1. Ducluzeau R, Ladire M, Raibaud P. (1984). Effect of bran ingestion on the microbial faecal floras of human donors and of recipient gnotobiotic mice, and on the barrier effects exerted by these floras against various potentially pathogenic microbial strains. Annales de Microbiologie 135A, 303-3 18.

    2. Fujiwara S, Hirota T, Nakazato H, Mizutani T, Mitsuoka T. (1991). Effect of konjac mannan on intestinal microbial metabolism in mice bearing human flora and in conventional F344 rats. Food and Chemical Toxicology 29, 601-606.

    3. Gordon HA, Pesti L. (1971). The gnotobiotic animal as a tool in the study of host microbial relationships. Bacteriological Reviews 35, 390429.

    4. Hazenberg MP, Bakker M, Verschoor-Burggraaf A. (1981). Effects of the human intestinal flora on germ-free mice. Journal of Applied Bacteriology 50, 95-106.

    5. Hill M. (1988). Gut flora and cancer in humans and laboratory animals. In: Rowland IR (ed) Role of the Gz4t Microflora in Toxicity and Cancer. Academic Press, London, pp. 461-502.

    6. Hirayama K, Kawamura S, Mitsuoka T. (1991). Development and stability of human faecal flora in the intestine of ex-germ-free mice. Microbial Ecology in Health and Disease 4, 95-99.

    7. Hirayama K, Misima M, Kawamura S, Itoh K, Takahashi E, Mitsuoka T. (1994). Effects of dietary supplements on the composition of fecal flora of human-flora-associated (HFA) mice. Bijidobacteria and Microflora 13, 1-7.

    8. Itoh K, Mitsuoka T. (1980). Production of gnotobiotic mice with normal physiological functions. I. Selection of useful bacteria from feces of conventional mice. Zeifschrift f i r Versuchstierkunde 22, 173-178.

    9. Itoh K, Ozaki A, Yamamoto T, Mitsuoka T. (1978). An autoclavable stainless steel isolator for small scale gnotobiotic experiments. Experimental Animals 27, 13-16.

    10. Mallett AK, Bearne CA, Rowland IR, Farthing MJG, Cole CB, Fuller R. (1987). The use of rats associated with a human faecal flora as a model for studying the effects of diet on the human gut microflora. Journal of Applied Bacteriology 63, 39-45.

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