Distribution of Black-Pigmented Prevotella and Porphyromonas species in the Dentition of Moderate Periodontitis Patients

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Isoshima, O. ; Ohta, H. ; Kurikara, H. ; Kato, K. ; Fukui, K. ; Murayama, Y. (2011)
  • Publisher: Microbial Ecology in Health and Disease
  • Journal: Microbial Ecology in Health and Disease (issn: 1651-2235, eissn: 1651-2235)
  • Related identifiers: doi: 10.3402/mehd.v8i4.8277
  • Subject:
    mesheuropmc: stomatognathic diseases | stomatognathic system

The distribution of Porphyromonas endodontalis, Porphyromonas gingivalis, Prevotella denticola, Prevotella intermedia, Prevotella loescheii, and Prevotella melaninogenica in periodontally healthy and diseased sites of patients was investigated. The periodontal pockets of two moderate periodontitis patients were scored clinically and sampled microbiologically at their first visit to Okayama University Dental Hospital. A total of 427 isolates of black-pigmented, anaerobic gram-negative rods (BPARs) were identified using a quick method of DNA-DNA hybridisation. In both subjects, all six BPAR species examined were distributed in clusters in certain areas of the dentition. P. gingivalis and Pr. intermedia were the most prevalent species identified. The DNA fingerprinting analysis of P. gingivalis isolates demonstrated that such a cluster was composed of one or two clonal types of the same species, and that five clonal types of the species were present in the whole dentition. For one subject, clinical and microbiological examinations were performed again 3 months later. P. gingivalis and Pr. intermedia were still prevalent and distributed in clusters. Among the five preexisting clonal types of P. gingivalis, four were still found, and six other types were recognised. Site-to-site and time-to-time variations in the species profile of BPAR and the clonal profile of P. gingivalis in individual sites may explain the uneven progression of disease in the dentition.Keywords: periodontal microflora, periodontitis, Porphyromonas gingivalis, Prevotella intermedia, DNA fingerprinting
  • References (51)
    51 references, page 1 of 6

    1 . Barua PK, Dyer DW, Neiders ME. (1990). Effect of iron limitation on Bacteroides gingivalis. Oral Microbiology and Immunology 5, 263-268.

    2. Bramanti TE, Holt SC. (1991). Roles of porphyrins and host iron transport proteins in regulation of growth of Porphyromonas gingivalis W50. Journal of Bacteriology 173, 7330-7339.

    3. Carman RJ, Ramakrishnan MD, Harper FH. (1990). Hemin levels in culture medium of Porphyromonas (Bacteroides) gingivalis regulate both hemin binding and trypsinlike protease production. Infection and Immunity 58, 4016-40 19.

    4. Chen C-K, Sunday GJ, Zambon JJ, Wilson ME. (1 990). Restriction endonuclease analysis of Eikenella corrodens. Journal of Clinical Microbiology 28, 1265-1270.

    5. Ezaki T, Hashimoto Y, Takeuchi N, Yamamoto H, Liu S, Miura H, Matsui K, Yabuuchi E. (1988). Simple genetic method to identify viridans group streptococci by colorimetric dot hybridization and fluorometric hybridization in microdilution wells. Journal of Clinical Microbiology 26, 1708-1713.

    6. Ezaki T, Takeuchi N, Liu S, Kai A, Yamamoto H, Yabuuchi E. (1988). Small-scale DNA preparation for rapid genetic identification of Campylobacter the growth and virulence of P. g i n g i v a l i ~ . ~ , ~ ~ ~sp~e,c~ies~ without radioisotope. Microbiology and Moreover, Shah and Gharbia41 and Kay et a1.I4 have demonstrated that some P. gingivalis strains produce haemolysins which might function in the release of haem from erythrocytes. Thus, in the case of P. gingivalis, the ability to produce haemolysins may confer a selective advantage over other haemrequiring bacteria and account for the observed predominance of this species in bleeding pockets. It has been noted by several investigators that there is considerable variability in the virulence of different P. gingivalis strains when they are injected into mice.9.30,4I9f this heterogeneity is also true for the P.gingivalis strains harboured in subject 1, the irregular pattern of active periodontal destruction in that subject may be explained by the distribution of such virulent strains. Further studies on the ability of different clonal types to produce abscesses in the mouse model are currently in progress in our laboratory. Immunology 32, 141-150.

    7. Ezaki T, Hashimoto Y, Yabuuchi E. (1989). Fluorometric deoxyribonucleic acid-deoxyribonucleic acid hybridization in microdilution wells as an alternative to membrane filter hybridization in which radioisotopes are used to determine genetic relatedness among bacterial strains. International Journal of Systematic Bacteriology 39, 224-229.

    8. Grenier D, Mayrand D. (1986). Nutritional relationships between oral bacteria. Infection and Immunity 53, 6 16-620.

    9. Grenier D, Mayrand D. (1987). Selected characteristics of pathogenic and nonpathogenic strains of Bacteroides gingivalis. Journal of Clinical Microbiology 25, 738-740.

    10. Hamilton IR, McKee AS, Bowden GH. (1989). Growth and metabolic properties of Bacteroides intermedius in anaerobic continuous culture. Oral Microbiology and Immunology 4, 89-97.

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