Contribution of Various Techniques of Molecular Analysis to Strain Identification of Candida glabrata

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Defontaine, A. ; Coarer, M. ; Bouchara, J. P. (2011)
  • Publisher: Microbial Ecology in Health and Disease
  • Journal: Microbial Ecology in Health and Disease (issn: 1651-2235, eissn: 1651-2235)
  • Related identifiers: doi: 10.3402/mehd.v9i1.8291
  • Subject:
    mesheuropmc: food and beverages | biochemical phenomena, metabolism, and nutrition | bacterial infections and mycoses | stomatognathic diseases

An evaluation of genetic typing systems (PFGE, mtDNA-RFLP, RAPD) on 22 isolates of Candida glabrata (ex Torulopsis glabrata) was undertaken. This evaluation indicated that PFGE has the highest degree of discrimination. The other methods can be used as complementary techniques when ambiguous results occur with PFGE.Keywords: Candida glabrata, mtDNA-RFLP, PFGE, RAPD, DNA polymorphism, epidemiology.
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