Development of Periodontal Microflora on Human Serum

Article English OPEN
Ter Steeg, P. F. ; Van Der Hoeven, J. S. (2011)
  • Publisher: Microbial Ecology in Health and Disease
  • Journal: Microbial Ecology in Health and Disease (issn: 1651-2235, eissn: 1651-2235)
  • Related identifiers: doi: 10.3402/mehd.v2i1.7428
  • Subject:
    mesheuropmc: food and beverages | stomatognathic diseases | stomatognathic system

This study was undertaken to identify the ecological niches of members of subgingival microflora. The succession of species during enrichment batch growth of subgingival plaque organisms in serum was monitored. Three phases could be distinguished during growth, firstly carbohydrate consumption by rapidly growing saccharolytic bacteria such as Eubacterium saburreum or Bifidobacterium adolescentis and Streptococcus spp. leading to lactic and formic acid production. Secondly a later phase in which protein was hydrolysed, some amino acid fermentation took place, remaining carbohydrates were used and lactate and formate were consumed; growth was dominated by Bacteroides intermedius or Bacteroides oralis, Veillonella parvula, Eubacterium spp. and Fusobacterium nucleatum, and a final phase characterised by progressive protein degradation and extensive amino acid fermentation by the predominant species, Peptostreptococcus micros and Eubacterium brachy. Toxic products such as sulphide, ammonia, butyric acid and other fatty acids accumulated.Keywords: Peptostreptococcus micros; Eubacterium; Bacteroides; Fusobacterium nucleatum; Periodontitis; Serum degradation; Niche; Growth-curve.
  • References (24)
    24 references, page 1 of 3

    1. Breure AM. (1 986). Hydrolysis and acidogenic fermentation of protein and carbohydrates in anaerobic waste water treatment. Ph.D. thesis, Univ. of Amsterdam, 7-20.

    2. Britz ML, Wilkinson R.G. (1982). Leucine dissimilation to isovaleric and isocaproic acids by cell suspensions of amino acid fermenting anaerobes: the Stickland reactions revisited. Canadian Journal of Microbiology 28,29 1-300.

    3. Carlsson J, Hotling JF, Sundqvist GK. (1984). Degradation of albumin, haemopexin, haptoglobin and transferrin, by black-pigmented Bacteroides species. Journal of Medical Microbiology 1 8 , 3 9 4 6 .

    4. Curtis MA, Kemp CW. (1984). Nitrogen metabolism in dental plaque. Cariology Today,Int. Congr. Zurich 1983, Karger, Basel, 212-222.

    5. Hazen MJ, Boogaard AEJM van den. (1982). Anaeroben en gaschromatografie I. Analyse, 355- 358.

    6. Hill GB, Ayers OM, Kohan AP. (1987). Characteristics and sites of infection of Eubacteriumnodatum. Eubacterium timidum, Eubacterium brachy, and other asaccharolytic Eubacteria. Journal of Clinical Microbiology 25, 154&1545.

    7. Hodge JE, Hofreiter BT. (1962). Determination of reducing sugars and carbohydrates. In: Whistler RL, Wolfram ML. (Eds) Methods in Carbohydrate Chemistry Vol 1, Academic Press, New York, 388-389.

    8. Hoeven JS van der, Franken HCM, Camp PJM, Dellebarre CW. (1978). Analysis of bacterial fermentation products by isotachophoresis. Applied and EnvironmentalMicrobiology 3 5 , 1 7 2 3 .

    9. Holdeman LV, Cat0 EP, Burmeister JA, Moore WEC. (1980). Description of Eubacterium timidum sp. nov., Eubacterium brachy sp. nov., and Eubacterium nodatum sp. nov. isolated from human periodontitis. International Journal of Systematic Bacteriology 30, 163-169.

    10. Holdeman LV, Cat0 EP, Moore WEC. (1977). Anaerobe laboratory manual, 4th ed. Blacksburg, Virginia, Virginia Polytechnic Institute and State University.

  • Metrics
    No metrics available
Share - Bookmark