Direct Automatic Bacterial Analysis of Rat Stool Samples; The Effects of Diet and Medical Treatment Studied by Computerised Gas-liquid Chromatography of Bacterial Fatty Acids

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Peltonen, R. ; Eerola, E. (2011)
  • Publisher: Microbial Ecology in Health and Disease
  • Journal: Microbial Ecology in Health and Disease (issn: 1651-2235, eissn: 1651-2235)
  • Related identifiers: doi: 10.3402/mehd.v5i2.7827

Gas-liquid chromatography (GLC) of bacterial cellular fatty acids was used to produce fatty acid profiles of the stool samples of six different groups of rats, three with different medications, two with experimental diets and a control group with ordinary laboratory food. Such a profile represents all the bacterial cellular fatty acids in the sample and thus reflects its microflora. The profiles were then compared to each other in order to test the applicability of the GLC method for simple and rapid detection, clustering and measurement of faecal bacterial changes. The analysis of the profiles was performed automatically by using coniputerised fatty acid profile correlation analysis combined with a cluster analysis. The results showed the method able to detect identical samples and quantify the differences between the groups. The experimental diets, fish-powder and all-meat, and the anltibiotics, ciprofloxacin and clindamycin, markedly altered the intestinal flora, whereas a non-steroidal anti-inflammatory analgesic, diclofenac, did not. The results indicate that the GLC of stool samples combined with computerised fatty acid profile analysis is a fast and useful method to detect and quantify changes in faecal floras induced by various environmental factors.Keywords: Faecal flora; Diet; Antimicrobe effect; Computer analysis; Gas chromatography; Fatty acids.
  • References (33)
    33 references, page 1 of 4

    1. Aries VC, Crowther BS, Drasar BS, Hill MJ, Ellis FR. (1971). The effect of a strict vegetarian diet on the faecal flora and faecal steroid concentration. Journal of Pathology 103,54-56.

    2. Cheng BO, Trimble RP, Illman RJ, Stone BA, Topping DL. (1987). Comparative effects of dietary wheat bran and its morphological components (aleurone and pericarp-seed coat) on volatile fatty acid concentrations in the rat. British Journal of Nutrition 51,69-76.

    3. Crowther JS, Drasar BS, Goddard P, Hill MJ, Johnson K . (1973). The effect of a chemically defined diet on the faecal flora and faecal steroid concentration. Gut 14,79&793.

    4. Eerola E, Lehtonen 0-P. (1988). Optimal data processing procedure for automatic bacterial identification by gas-liquid chromatography of cellular fatty acids. Journal of Clinical Microbiology 26, 1745-1 753.

    5 . Evaldson G, Heimdahl A, Kager L, Nord CE. (1 982). The normal human anaerobic microflora. Scandinavian Journal of Infectious Diseases Supplementum 35,9-15.

    6. Finegold SM,Attebery HR, Sutter VL. (1974).Effect of diet on human fecal flora: comparison of Japanese and American diets. American Journal of Clinical Nutrition 27, 14561469.

    7. Finegold SM, Sutter VL, Sugihara PT, Elder HA. Lehmann SM, Philips RL. (1977). Fecal microbial flora in Seventh-Day Adventist populations and control subjects. American Journal of Clinical Nutrition 30,1781-1792.

    8. Goldin BR, Gorbach SL. (1984). The effect of milk and lactobacillus feeding on human intestinal bacterial enzyme activity. American Journal of Clinical Nutrition 39, 7 5 6 7 6 I .

    9. Goldin BR, Swenson L, Dwyer J, Sexton M, Gorbach SL. (1980). Effect of diet and Lactobacillus acidophilus supplements on human fecal bacterial enzymes. Journal of National Cancer Institute 64, 255-261.

    10. Hentges DJ. (1980). 'Does diet influence human fecal microflora composition? Nutrifion Review 38, 329-3 36.

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