In vitro Adhesion and Ribotypes Among Oral Bacteria Isolated From Plaque on Titanium, Hydroxyapatite and Amalgam Surfaces

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Leonhardt, Å. ; Olsson, J. ; Dahlén, G. (2011)
  • Publisher: Microbial Ecology in Health and Disease
  • Journal: Microbial Ecology in Health and Disease (issn: 1651-2235, eissn: 1651-2235)
  • Related identifiers: doi: 10.3402/mehd.v8i6.8321
  • Subject:
    mesheuropmc: stomatognathic diseases | stomatognathic system

The aim of this study was to examine initial bacterial adherence to saliva coated titanium, hydroxyapatite, and amalgam surfaces and to determine whether different bacterial geno-or phenotypic variants were attracted to these surfaces. From six individuals, each with three different test surfaces, 18 strains each of Actinomyces naeslundii, Streptococcus sanguis, S. oralis and S. mitis were investigated by rRNA gene restriction analysis (ribotyping), to disclose potential genotypic similarities within each species isolated from one person. Experimentally, 18 strains each of S. sanguis, S. oralis, S. mutans, S. salivarius, A. naeslundii, six of Fusobacterium nucleatum, and nine strains of black-pigmented Prevotella spp. were tested for adhesion in vitro to the same surfaces coated with saliva from the six individuals. All strains adhered in a higher degree to saliva coated test surfaces than to albumin coated control surfaces, although this tendency was significantly lower for S. salivarius than for other streptococcal species or A. naeslundii. No distinct pattern of ribotypes or bacterial adhesion subtypes were found to be attracted to the test surfaces. Titanium test surfaces were not colonised by bacterial adhesion subgroups different from those which in the same individual colonised hydroxyapatite or amalgam surfaces.Keywords: adhesion, amalgam, dental plaque, hydroxyapatite, oral microbiota, ribotypes, titanium.
  • References (31)
    31 references, page 1 of 4

    I . Adell R, Lekholm U, Rockler B, Brinemark P-I. (1981). A 15-year study of osseointegrated implants in the treatment of the edentulous jaw. International Journal of Oral Surgery 10, 387416.

    2. Adell R. (1985). Long-term treatment results. In: Brinemark P-I, Zarb G, Albrektsson T (eds) Tissue Integrated Prosthesis. Osseointegration in Clinical Dentistry. Quintessence Publishing, Chicago, pp. 175-186.

    3. Albrektsson T, Brinemark P-I, Hansson HA, Lindstrom J. (1981). Osseointegrated titanium implants. Requirements for ensuring a long-lasting, direct bone-to-implant anchorage in man. Acta Orthopedica Scandinavica 52, 155-1 70.

    4. Bowden G , Johnson J, Schachtele C. (1993). Characterization of Actinomyces with genomic DNA fingerprints and rRNA gene probes. Journal of Dental Research 72, 1171-1 179.

    5. Christensen GD, Baddour LM, Hasty DL, Lowrance JH, Simpson WA. (1989). Microbial and foreign body factors in the pathogenesis of medical device infections. In: Bisno AL, Waldvogel FA (eds) Infections Associated with Indwelling Medical Devices. American Society for Microbiology, Washington, DC, pp. 27-59.

    6. Clark WB, Bammann LL, Gibbons RJ. (1978). Comparative estimates of bacterial affinities and adsorption sites on hydroxyapatite surfaces. Infection and Immun~ty19, 846-853.

    7. Ellingsen JE. (1991). A study on the mechanism of protein adsorption to TiO,. Biomaterials 12, 593-596.

    8. Fillery ED, Bowden GH, Hardie JM. (1978). A comparison of strains of bacteria designated Actinomyces viscosus and Actinomyces naeslundii. Caries Research 12, 299-312.

    9. Genco RJ, Loos BG. (1991). The use of genomic DNA fingerprinting in studies of the epidemiology of bacteria in periodontitis. Journal of Clinical Periodontology 18, 396405.

    10. Gibbons RJ, van Houte J. (1975). Bacterial adherence in oral microbial ecology. Annual Review of Microbiology 29, 1 9 4 4 .

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