Isolation and prevalence of Simonsiella sp. in lesions of erosive lichen planus and on healthy human oral mucosa

Article English OPEN
Pankhurst, C. L. ; Auger, D. W. ; Hardie, J. M. (2011)
  • Publisher: Microbial Ecology in Health and Disease
  • Journal: Microbial Ecology in Health and Disease (issn: 1651-2235, eissn: 1651-2235)
  • Related identifiers: doi: 10.3402/mehd.v1i1.7384
  • Subject:
    mesheuropmc: integumentary system | stomatognathic diseases | stomatognathic system

Members of the genus Simonsiella are aerobic Gram-negative segmented multicellular filamentous bacteria found in the oral cavity. There is a wide variation in the reported prevalence of Simonsiella in the oral cavity of man (0.5 per cent to 32 per cent). The aim of the investigation was to determine the prevalence of Simonsiella at different sites in the healthy oral mucosa of 100 dental patients and to compare this with isolates from lesions of 50 patients with oral erosive lichen planus. Oral swabs were inoculated at the chairside onto a complex serum enriched agar. Utilising the unique morphological characteristics, identification was made by direct microscopic examination of culture plates. The morphology and ultrastructure were further investigated by scanning and transmission electron microscopy. Simonsiella was isolated from 22 per cent of patients at one or more sites, with the dorsum of the tongue being the most frequently colonised area. The 50 patients with erosive lichen planus yielded only two positive cultures from the site of the lesion and in one of these Simonsiella was also isolated from the healthy site. In the remaining 48 patients, two were positive at non-lesion sites only. No strong association between Simonsiella and lesions of erosive lichen planus could be demonstrated in this study.Keywords: Simonsiella; oral flora; erosive lichen planus; morphology; ultrastructure
  • References (9)

    1. Bruckner R. J., Fahey S. H. (1969).A giant bacterial form (Simonsiella) seen in oral exfoliative cytology preparations. Oral Surgery, Oral Medicine and Oral Pathology 28, 197-20 1.

    2. Carandina G., Bacchelli M., Virgili A., Strumia. 1984. Simonsiella filaments isolated from erosive lesions of the human oral cavity. Journal of Clinical Microbiology 19,931-933.

    3. Gregory D. A.,Kuhn D. A., Daly K. R., Flygenning K. (1985). Statistical association of dietary components with Simonsiella species residing in normal human mouths. Applied and Environmental Microbiology 50,704-705.

    4. Jenkins C. L., Kuhn D. A., Daly K. R. (1977).Fatty acid composition of Simonsiella strains. Archives of Microbiology 113,209-213.

    5. Kuhn D. A,, Gregory D. A,, Pangborn J., Mandel M. (1974). Simonsiella strains from the human oral cavity.Journal of Dental Research 53 (special issue), 108.

    6. Kuhn D. A , ,Gregory,D. A.,Buchanan G. E.,Nyby M. D., Daly K. R. (1978). Isolation, characterisation and numerical taxonomy of Simonsiella strains from the oral cavities of cats, dogs, sheep and humans. Archives of Microbiology 118,235-241.

    7. McCowan R. P., Cheng K . J., Costerton J. W. (1979). Colonisation of a portion of the bovine tongue by unusual filamentous bacteria. Applied and Environmental Microbiology 37, 1224-1229.

    8. Pangborn J., Kuhn D. A.,Woods J. R. (1977). Dorsal-ventral differentiation in Simonsiella and other aspects of its morphology and ultrastructure. Archives of Microbiology 113, 197-204.

    9. Steed P. M . (1963). Simonsiellaceae fam. nov. with characterisation of Simonsiella crassa and Alysiella jil$ormis. Journal of General Microbiology 29, 615-624. Whitehouse R. L. S., Jackson H . , Jackson M . C., Ramji M. M. (1987). Isolation of Simonella sp. from a neonate. Journal of Clinical Microbiology 25, 522-525.

  • Similar Research Results (2)
  • Metrics
    No metrics available
Share - Bookmark