publication . Article . Other literature type . 2010

Crosstalk between mRNA 3′ End Processing and Transcription Initiation

Søren Lykke-Andersen; Jørgen Kjems; Torben Heick Jensen; Edouard Bertrand; Christophe K. Mapendano;
Open Access English
  • Published: 01 Nov 2010
  • Publisher: HAL CCSD
  • Country: France
Transcription and mRNA maturation are interdependent events. Although stimulatory connections between these processes within the same round of transcription are well described, functional coupling between separate transcription cycles remains elusive. Comparing time-resolved transcription profiles of single-copy integrated beta-globin gene variants, we demonstrate that a polyadenylation site mutation decreases transcription initiation of the same gene. Upon depletion of the 3' end processing and transcription termination factor PCF11, endogenous genes exhibit a similar phenotype. Readthrough RNA polymerase II (RNAPII) engaged on polyadenylation site-mutated tran...
free text keywords: Base Sequence Cyclin-Dependent Kinase 9/metabolism HEK293 Cells Humans Models, Messenger/genetics/*metabolism TATA-Box Binding Protein/metabolism Time Factors *Transcription, Biological Molecular Sequence Data Phenotype Phosphorylation Phosphoserine/metabolism Point Mutation/genetics Poly A/genetics Promoter Regions, Genetic/genetics RNA 3' End Processing/*genetics RNA Polymerase II/metabolism RNA Splicing/genetics RNA, Genetic mRNA Cleavage and Polyadenylation Factors/metabolism, [SDV.BBM]Life Sciences [q-bio]/Biochemistry, Molecular Biology, Cell Biology, Molecular Biology, Transcription factor II A, Transcription factor II B, RNA polymerase II, biology.protein, biology, Response element, Genetics, General transcription factor, Promoter, TAF2, E-box
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