publication . Article . Other literature type . 2015

Agrobacterium Tumefaciens Mediated Transformation of rolC::Hd3a-GFP in Black Rice (Oryza Sativa L. cv. Cempo Ireng) to Promote Early Flowering☆

Purwestri, Yekti Asih; Sari, Resta Dewi Komala; Anggraeni, Lisa Novita; Sasongko, Aries Bagus;
Open Access
  • Published: 01 Jan 2015 Journal: Procedia Chemistry, volume 14, pages 469-473 (issn: 1876-6196, Copyright policy)
  • Publisher: Elsevier BV
Abstract
Abstract The purpose of this study was to transform rolC::Hd3a-GFP by Agrobacterium tumefaciens on black rice (Oryza sativa L. cv. Cempo Ireng). The callus formation were induced from scutellum using two different media, 2N6 and 2,4-dichlorophenoxyacetic acid (2,4-D). PCR analysis using specific primers for Hd3a and hpt was performed to determine the stability of rolC::Hd3a-GFP construct before co-cultivation of callus and Agrobacterium tumefaciens. This study indicated that black rice callus induction on 2N6 medium responded faster than that on MS 2,4-D medium and generated friable calli for Agrobaterium-mediated transformation. The screening result showed that...
Subjects
Medical Subject Headings: fungifood and beverages
free text keywords: Chemistry(all), Chemical Engineering(all), Black rice (Oryza sativa L. cv. Cempo Ireng), Hd3a, hpt, 2N6 medium, Agrobacterium-mediated transformation., General Chemistry, General Chemical Engineering, Oryza sativa, Scutellum, Black rice, Green fluorescent protein, Botany, 2,4-Dichlorophenoxyacetic acid, chemistry.chemical_compound, chemistry, Callus, Agrobacterium tumefaciens, biology.organism_classification, biology, Agrobacterium, Analytical chemistry
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