publication . Article . Other literature type . 2014

Generation and molecular characterization of a monoclonal antibody reactive with conserved epitope in sphingomyelinases D from Loxosceles spider venoms.

C. Dias-Lopes; L. Felicori; L. Rubrecht; S. Cobo; L. Molina; C. Nguyen; P. Galéa; C. Granier; F. Molina; C. Chávez-Olortegui;
Open Access English
  • Published: 01 Apr 2014
  • Publisher: HAL CCSD
  • Country: France
Abstract
Abstract We report the production of a neutralizing monoclonal antibody able to recognize the venoms of three major medically important species of Loxosceles spiders in Brazil. The mAb was produced by immunization of mice with a toxic recombinant L. intermedia sphingomyelinase D {SMases D isoform (rLiD1)} [1] and screened by enzyme-linked immunosorbent assay (ELISA) using L. intermedia , L. laeta and L. gaucho venoms as antigens. One clone (LiD1mAb16) out of seventeen anti-rLiD1 hybridomas was cross-reactive with the three whole Loxosceles venoms . 2D Western blot analysis indicated that LiD1mAb16 was capable of interacting with 34 proteins of 29–36 kDa in L. in...
Subjects
free text keywords: [SDV]Life Sciences [q-bio], Loxosceles spider venoms, Sphingomyelinases D, Monoclonal antibody, Epitopes, Public Health, Environmental and Occupational Health, Immunology and Microbiology(all), Molecular Medicine, veterinary(all), Infectious Diseases, General Immunology and Microbiology, General Veterinary, Molecular biology, Epitope, Linear epitope, medicine.drug_class, medicine, Spider Venoms, Loxoscelism, medicine.disease, Immunology, Antibody, biology.protein, biology, Antigen, Western blot, medicine.diagnostic_test
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