publication . Article . Other literature type . 2001

Microarray identification of FMRP-associated brain mRNAs and altered mRNA translational profiles in fragile X syndrome.

Brown, Victoria; Jin, Peng; Ceman, Stephanie; Darnell, Jennifer C.; O'Donnell, William T.; Tenenbaum, Scott A.; Jin, Xiaokui; Feng, Yue; Wilkinson, Keith D.; Keene, Jack D.; ...
Open Access
  • Published: 01 Nov 2001 Journal: Cell, volume 107, issue 4, pages 477-487 (issn: 0092-8674, Copyright policy)
  • Publisher: Elsevier BV
Abstract
AbstractFragile X syndrome results from the absence of the RNA binding FMR protein. Here, mRNA was coimmunoprecipitated with the FMRP ribonucleoprotein complex and used to interrogate microarrays. We identified 432 associated mRNAs from mouse brain. Quantitative RT-PCR confirmed some to be >60-fold enriched in the immunoprecipitant. In parallel studies, mRNAs from polyribosomes of fragile X cells were used to probe microarrays. Despite equivalent cytoplasmic abundance, 251 mRNAs had an abnormal polyribosome profile in the absence of FMRP. Although this represents <2% of the total messages, 50% of the coimmunoprecipitated mRNAs with expressed human orthologs were...
Subjects
free text keywords: Biochemistry, Genetics and Molecular Biology(all), General Biochemistry, Genetics and Molecular Biology, FMR1, Microarray, Genetics, Molecular biology, Polysome, Fragile X syndrome, medicine.disease, medicine, RNA, Messenger RNA, DNA microarray, Biology, RNA-binding protein
Related Organizations
Abstract
AbstractFragile X syndrome results from the absence of the RNA binding FMR protein. Here, mRNA was coimmunoprecipitated with the FMRP ribonucleoprotein complex and used to interrogate microarrays. We identified 432 associated mRNAs from mouse brain. Quantitative RT-PCR confirmed some to be >60-fold enriched in the immunoprecipitant. In parallel studies, mRNAs from polyribosomes of fragile X cells were used to probe microarrays. Despite equivalent cytoplasmic abundance, 251 mRNAs had an abnormal polyribosome profile in the absence of FMRP. Although this represents <2% of the total messages, 50% of the coimmunoprecipitated mRNAs with expressed human orthologs were...
Subjects
free text keywords: Biochemistry, Genetics and Molecular Biology(all), General Biochemistry, Genetics and Molecular Biology, FMR1, Microarray, Genetics, Molecular biology, Polysome, Fragile X syndrome, medicine.disease, medicine, RNA, Messenger RNA, DNA microarray, Biology, RNA-binding protein
Related Organizations
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