publication . Article . 2006

Chimeric HIV-1 containing SIV matrix exhibit enhanced assembly in murine cells and replicate in a cell-type-dependent manner in human T cells

Ping Chen; Wolfgang Hübner; Kareen Riviere; Yu-Xin Liu; Benjamin K. Chen;
Open Access
  • Published: 01 May 2006 Journal: Virology, volume 349, issue 1, pages 1-12 (issn: 0042-6822, Copyright policy)
  • Publisher: Elsevier BV
Abstract
AbstractMurine fibroblasts expressing viral receptors and human cyclin T1 allow HIV-1 entry and viral gene expression but do not support efficient assembly. A chimeric HIV-1 carrying a non-homologous matrix (MA) from murine leukemia virus in place of HIV-1 MA can assemble efficiently in murine cells, yet has poor infectivity. Here, we assess the ability of a homologous MA from SIV MAC239 to complement assembly and infection in chimeric viruses designated SHIV(MA). The resulting SHIV(MA) chimeras produce more virus than native HIV-1 when transfected into murine cells. SHIV(MA) exhibits cell-type-specific replication in human T cell lines, replicating well in MT4 ...
Subjects
Medical Subject Headings: virusesvirus diseases
free text keywords: Virology, Retrovirus, Human immunodeficiency virus type 1, Simian immunodeficiency virus, p17 matrix protein, gp160 Env protein, Virus assembly, Murine leukemia virus, biology.organism_classification, biology, Pseudotyping, Chimera (genetics), Transfection, medicine.disease_cause, medicine, Jurkat cells, T cell, medicine.anatomical_structure
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