publication . Article . 1997

Alpha- and beta-subunits of a V-type membrane ATPase in a hyperthermophilic sulfur-dependent archaeum, Thermococcus sp. KI

Iida, Toshii; Kanai, Satoru; Inatomi, Ken-ichi; Kamagata, Yoichi; Maruyama, Tadashi;
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  • Published: 01 Oct 1997 Journal: Biochimica et Biophysica Acta (BBA) - Biomembranes, volume 1,329, pages 12-17 (issn: 0005-2736, Copyright policy)
  • Publisher: Elsevier BV
Abstract
Abstract The genes encoding α- and β-subunits of a V-type ATPase in a sulfur-dependent hyperthermophilic archaeum, Thermococcus sp. KI, were cloned and sequenced. The deduced amino acid sequences were approximately 60, 50 and 25% identical to those of other archaeal, eukaryotic V-type and E. coli F-type ATPase, respectively. Phylogenetic analysis revealed that Thermococcus ATPase was closely related to that of Thermus, and those of Methanosarcina and Halobacterium.
Subjects
free text keywords: Biophysics, Cell Biology, Biochemistry, Amino acid, chemistry.chemical_classification, chemistry, ATPase, biology.protein, biology, Molecular biology, Molecular cloning, Hyperthermophile, Halobacterium, biology.organism_classification, Methanosarcina, Thermus, Thermococcus, Membrane ATPase, Gene cloning, Archaeum, Phylogenetic tree, (Thermococcus)
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