Micropropagation of Chrysanthemum leiophyllum (Asteraceae)

Article Russian OPEN
Kuritskaya, E. V. ; Nedoluzhko, A. I. ; Vrzhosek, E. V. ; Boltenkov, E. V. (2016)
  • Publisher: Altai State University
  • Journal: Turczaninowia (issn: 1560-7267, eissn: 1560-7259)

The East Asian species Chrysanthemum leiophyllum is a source of adaptive traits in the breeding of Ch. × morifolium. The specifics of introduction of Ch. leiophyllum into an in vitro culture and its micropropagation were studied. Stem segments with axillary buds were used as explants. A technique for sterilization of stems to obtain viable explants was developed. Shorter and vitrified shoots, with the callus tissue developing in their basal part, formed on Murashige and Skoog (MS) medium supplemented with 0,5 mg/L 6-benzylaminopurine (BAP) and 30 g/L sucrose. A decrease in the BAP level to 0.2 mg/L promoted shoot growth. A two-fold decrease in the sucrose concentration raised the multiplication coefficient of Ch. leiophyllum shoots to 10,3, while a two-fold decrease in the macro-salts concentration raised the multiplication coefficient to11.7. It was found that concentration of macro-salts has an effect on shoot development. The medium with half-strength MS macrosalts in combination with 0.2 mg/L BAP was optimal for shoot propagation. Adventitious buds formed in the basal part of shoots. Roots developed intensively in the medium with half-strength MS macrosalts and in the presence of 0,5 mg/L indoleacetic acid (IAA) and 30 g/L sucrose. Growth of roots was influenced mostly by plant growth regulators (PGR); the optimal PGR at the rooting stage was IAA. The efficiency of adaptation of plants to soil conditions constituted 98 %. The plants obtained in vitro and transplanted to soil grew normally. The ground-hugging rosettes of leaves developed by the end of the first year on the open soil; in the second year, the plants entered the generative phase. The chromosome number was 2n = 36 both in the in vitro obtained plants and in the original plant.Key words: Chrysanthemum leiophyllum, in vitro culture, organogenesis,phytohormones, chromosome number.
  • References (14)
    14 references, page 1 of 2

    Bush S. R., Earle E. D, Langhans R. W. 1976. Plantlets from petal segments, petal epidermis, and shoot tips of the periclinal chimera, Chrysanthemum morifolium 'Indianapolis'. Amer. J. Bot. 63(6): 729-737. DOI: 10.2307/2442032

    Chakrabarty D., Mandal A. K. A., Datta S. K. 1999. Management of chimera through direct shoot regeneration from florets of chrysanthemum ( Chrysanthemum morifolium Ramat.). J. Hort. Sci. Biotechnol. 74(3): 293-296. DOI: 10.1080/14620316.1999.11511111

    Chakrabarty D., Mandal A. K. A., Datta S. K. 2000. SEM and light microscopic studies on direct shoot regeneration from ray florets of Chrysanthemum. Israel J. Plant Sci. 48(2): 105-107. DOI: 10.1560/B6WM-2565-2BVY-P3W9

    Hill G. P. 1968. Shoot formation in tissue cultures of Chrysanthemum 'Bronze Pride'. Physiol. Plant. 21(2): 386-389. DOI: 10.1111/j.1399-3054.1968.tb07262.x

    Karim M. Z., Amin M. N., Azad M. A. K., Begum F., Rahman M. M., Ahmad S., Alam R. 2003. In vitro shoot multiplication of Chrysanthemum morifolium as affected by sucrose, agar and pH. Biotechnology 2(2): 115-120. DOI: 10.3923/biotech.2003.115.120

    Karp A. 1995. Somaclonal variation as a tool for crop improvement. Euphytica 85(1): 295-302. DOI 10.1007/ BF00023959

    Kaul V., Miller R. M., Hutchinson J. F., Richards D. 1990. Shoot regeneration from stem and leaf explants of Dendranthema grandiflora Tzvelev (syn. Chrysanthemum morifolium Ramat.). Plant Cell Tissue Organ Cult. 21(1): 21-30. DOI: 10.1007/BF00034487

    Khalid N., Davey M. R., Power J. B. 1989. An assessment of somaclonal variation in Chrysanthemum morifolium: the generation of plants of potential commercial value. Sci. Hort. 38(3-4): 287-294. DOI: 10.1016/0304- 4238(89)90076-9

    Lee S. M., Lee C. H., Kim K. S. 2004. Multiplication of Dendranthema zawadskii ssp. latilobum var. leiophyllum through multiple shoot induction from rhizome-derived shoot tip culture. J. Korean Soc. Hort. Sci. 45(1): 43-48.

    Lu C. Y, Nugent G., Wardley T. 1990. Efficient, direct plant regeneration from stem segments of chrysanthemum (Chrysanthemum morifolium Ramat. cv. Royal Purple). Plant Cell Rep. 8(12): 733-736. DOI: 10.1007/BF00272106.

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