DEVELOPMENT AND VALIDATION OF IDENTIFICATION METHOD OF FLAVONOIDS IN THE MARIGOLD TINCTURE. MESSAGE 1.

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Хохлова (Khokhlova), Екатерина (Ekaterina) Александровна (Aleksandrovna) ; Здорик (Zdoryk), Александр (Aleksandr) Анатольевич (Anatol'evich) ; Георгиянц (Georgiyants), Виктория (Viktorija) Акоповна (Akopovna) (2015)
  • Publisher: Altai State University
  • Journal: Khimiia rastitel'nogo syr'ia (Chemistry of plant raw material) (issn: 1029-5143, eissn: 1029-5151)
  • Related identifiers: doi: 10.14258/jcprm.201501397
  • Subject: настойка календулы; флавоноиды; тонкослойная хроматография; валидация | Marigold tincture, flavonoids, thin-layer chromatography, validation

The thin-layer chromatography method of flavonoids’ identification in the Marigold tincture was developed. The validation characteristics such as specificity, robustness, precision were studied. During robustness study the influences of chamber’s type, stationary phase, development distance, conditions of dryness and derivatization, stability of the test solution during analysis were investigated. The optimal conditions of chromatography analysis were investigated. The specificity of method of flavonoids’ determination was proved with the reference standard rutin, Marigold tincture, prepared from authentic herbal raw material, and the adulteration. The precision of the method on the same and on the different plates, the interlaboratory precision were studied. The possibility of usage developed method for standardization of Marigold tincture in the condition of the quality control lab was proved. For assuring specificity of identification of Marigold tincture, the determination of characteristic substances for Marigold flower – calendulosides, is reasonable. 
  • References (13)
    13 references, page 1 of 2

    1. Gosudarstvennyi reestr lekarstvennykh sredstv Ukrainy po sostoianiiu na 08.04.2014 g. [State Register of Medicinal Products of Ukraine (04.08.2014)]. Kiev, 2014. URL: http://www.drlz.kiev.ua. (in Russ.).

    2. Zdoryk O.A., Khokhlova K.O., Savchenko L.P., Georgiyants V.A. The 17-th International Congress Phytopharm 2013. Vienna, Austria, 8-10 July 2013, pp. 51.

    3. Zdoryk O. ., Khokhlova K.O., Georgiyants V. ., Vyshnevska L.I. International Journal of Pharmaceutical Compounding, 2014, no. 3, pp. 248-252.

    4. Gosudarstvennaia farmakopeia Ukrainy. [State Pharmacopoeia of Ukraine]. Kharkiv, 2008, 1 edition, addition 2. 620 p. (in Russ.).

    5. Gosudarstvennaia farmakopeia Ukrainy. [State Pharmacopoeia of Ukraine]. Kharkiv, 2011. 1 edition, addition 4. 540 p. (in Russ.).

    6. Khokhlova E.A., Vishnevs'ka L.I., Garna S.V., Kotov A.G. Farmakom, 2013, no. 1, pp. 38-51. (in Russ.).

    7. Khokhlova E.A. Razrabotka i standartizatsiia nastoiki «Aterofit-norma» dlia terapii ishemicheskoi bolezni serdtsa : dis. … kand. farmats. nauk. [Development and standardization of infusion "Aterofit norm" for the treatment of coronary heart disease: the dissertation of the candidate of pharmaceutical sciences]. Kharkiv, 2013, 216 p. (in Russ.).

    8. Reich E., Schibli A. High-Performance Thin-Layer Chromatography for the Analysis of Medicinal Plants. New York, 2006, 264 p.

    9. Koll K., Reich E., Blatter A., Veit M. Journal of AOAC International, 2003, vol. 86, no. 5, pp. 909-915.

    10. Kurkin V.A., Sharova O.V. Khimiia prirodnykh soedinenii, 2007, no. 2, pp. 179-180. (in Russ.).

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