DEVELOPMENT AND VALIDATION OF IDENTIFICATION METHOD OF FLAVONOIDS IN THE MARIGOLD TINCTURE. MESSAGE 1.
Хохлова (Khokhlova), Екатерина (Ekaterina) Александровна (Aleksandrovna)
Здорик (Zdoryk), Александр (Aleksandr) Анатольевич (Anatol'evich)
Георгиянц (Georgiyants), Виктория (Viktorija) Акоповна (Akopovna)
- Publisher: Altai State University
Khimiia rastitel'nogo syr'ia (Chemistry of plant raw material)
(issn: 1029-5143, eissn: 1029-5151)
настойка календулы; флавоноиды; тонкослойная хроматография; валидация | Marigold tincture, flavonoids, thin-layer chromatography, validation
The thin-layer chromatography method of flavonoids’ identification in the Marigold tincture was developed. The validation characteristics such as specificity, robustness, precision were studied. During robustness study the influences of chamber’s type, stationary phase, development distance, conditions of dryness and derivatization, stability of the test solution during analysis were investigated. The optimal conditions of chromatography analysis were investigated. The specificity of method of flavonoids’ determination was proved with the reference standard rutin, Marigold tincture, prepared from authentic herbal raw material, and the adulteration. The precision of the method on the same and on the different plates, the interlaboratory precision were studied. The possibility of usage developed method for standardization of Marigold tincture in the condition of the quality control lab was proved. For assuring specificity of identification of Marigold tincture, the determination of characteristic substances for Marigold flower – calendulosides, is reasonable.