BIOACTIVE CONSTITUENTS FROM SEA BUCKTHORN HIPPOPHAE RHAMNOIDES L. TREE GREEN

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Кукина (Kukina), Татьяна (Tat'jana) Петровна (Petrovna) ; Щербаков (Shherbakov), Дмитрий (Dmitrij) Николаевич (Nikolaevich) ; Геньш (Gen'sh), Константин (Konstantin) Викторович (Viktorovich) ; Тулышева (Tulysheva), Екатерина (Ekaterina) Анатольевна (Anatol'evna) ; Сальникова (Sal'nikova), Ольга (Ol'ga) Иосифовна (Iosifovna) ; Гражданников (Grazhdannikov), Александр (Aleksandr) Евгеньевич (Evgen'evich) ; Колосова (Kolosova), Евгения (Evgenija) Андреевна (Andreevna) (2016)
  • Publisher: Altai State University
  • Journal: Khimiia rastitel'nogo syr'ia (Chemistry of plant raw material) (issn: 1029-5143, eissn: 1029-5151)
  • Related identifiers: doi: 10.14258/jcprm.2016011100
  • Subject: Hippophae rhamnoides L., polyprenols, dolichols, sterols, triterpene alcohols, triterepenovye acid, leafy shoots | Hippophae rhamnoides L., полипренолы, долихолы, стерины, тритерпеновые спирты, тритерепеновые кислоты, облиствен-ные побеги

The data about twigs with leaves constituents of sea buckthorn are insufficient in spite of the fact that it is the large waste product of sea buckthorn oil production and tender cutting. Twigs with leaves (so called tree green) were object of our investigation. We used hexane as extragent. Hexane is an analog of commercial solvent and guarantees exhaustive extraction of lipophilic constituents. The composition of this extract was investigated by GC/MS and HPLC. As a result 67 neutral and 29 acid constituents such as aliphatic and triterpenic acids and alcohols, sterols, aldehydes, polyprenols, dolichols, hydrocarbons were identified. The main component of the sterol fraction - β-sitosterol. Its content – 6,9% by weight of the extract, which is significantly higher than the ether extracts of sea buckthorn leaves and fruit pulp. In the extract it is found predominantly in the free form. The acidic fraction along with main aliphatic acids contain minor highly triterpene acids (up to 5% weight of the extract). The main component of dolichol and polyprenol fraction (to 4,2%) is a compaunds with 11 and 17 isoprene units. These results suggest leafy shoots of sea buckthorn promising source of biologically active compounds.
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