СПОСОБ ПОЛУЧЕНИЯ ЛЕКАРСТВЕННОГО РАСТИТЕЛЬНОГО СЫРЬЯ ЛАПЧАТКИ БЕЛОЙ (POTENTILLA ALBA L.) В УСЛОВИЯХ ГИДРОПОНИКИ

Article Russian OPEN
Тихомирова (Tikhomirova), Людмила (Liudmila) Ивановна (Ivanovna) ; Базарнова (Bazarnova), Наталья (Natal'ia) Григорьевна (Grigor'evna) ; Ильичёва (Il'icheva), Татьяна (Tat'iana) Николаевна (Nikolaevna) ; Сысоева (Sysoeva), Александра (Aleksandra) Викторовна (Viktorovna) (2016)
  • Publisher: Altai State University
  • Journal: Khimiia rastitel'nogo syr'ia (Chemistry of plant raw material) (issn: 1029-5143, eissn: 1029-5151)
  • Related identifiers: doi: 10.14258/jcprm.2016031228
  • Subject: Potentilla alba L.; the technology of micropropagation; medicinal plant raw; intact plants; plant regenerants; biological activity | Potentilla alba L.; технология микроклонального размножения; лекарственное растительное сырье; интактные растения; растения-регенеранты; биологическая активность
    mesheuropmc: food and beverages

The aim of this work was to develop a biotechnological method of producing raw material P. alba, preserving the group chemical composition and biological activity comparable to the intact plants. For the production of renewable raw materials P. alba developed the technology of micropropagation and cultivation in the conditions of hydroponics. Vegetative buds of shoots in the spring was separated from rhizomes. The sterilization was conducted under the conditions of laminarbox 0,1% solution of mercuric chloride for 10 minutes. Nutrient medium was prepared according to the recipe MS containing of 1,0-2,5 µm BAP. After 20–30 days the developed shoots were transferred to propagation medium containing 0,5-1,0 µm BAP+0,25-0,5 µm IAA+0,05 microns GK. Microdamage were planted on MS medium supplemented with 3 ám NAA.The cultivation of vegetable raw materials was carried out in a nutrient solution based on liquid MS containing composition macrosoma¼, ¼ part microsoma, full set of vitamins, chelate iron, and calcium chloride. Vegetative mass was obtained at a temperature of 24–26 °C, light regime: 16 hours day 8 hours night. During year-round cultivation of the biomass of medicinal plants of the white cinquefoil on this method in the conditions of a hydroponic set-up "Minivet" was 10,05 kg/m2 in wet weight for one year.In extracts of intact plants of P. alba and of regenerated plants according to the results of the qualitative responses identified flavonoids, tannins (hydrolyzable tannins and condensed).Extracts of regenerated plants of P. alba exhibit biological activity against herpes virus is comparable to the intact plants.It is necessary to continue studies of the chemical composition of renewable raw materials P. alba and determining the biological activity of the extracts against the most dangerous viruses to humans.
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