EX VITRO ADAPTATION OF REGENERATED STRAWBERRY (MOSCOW DELICACY VARIETY)

Article Russian OPEN
Borodulina, I. D. ; Plaksina, T. V. (2015)
  • Publisher: Altai State University, Biological Faculty
  • Journal: Acta Biologica Sibirica (issn: 2412-1908, eissn: 2412-1908)
  • Related identifiers: doi: 10.14258/abs.v1i1-2.832
  • Subject: Biology; Ecology; Botany | regenerates; ex vitro adaptation; plant culturing unit; chitosan sulphate solution; ultrasound. | Биология; Экология; Ботаника | регенеранты; адаптация к условиям ex vitro; гидропонная установка; раствор сульфата хитозана; ультразвук.

Adaptation of regenerated plants of strawberry (varietу Moscow Delicaсy) towards conditions in vitro were performed on a hydroponic installation "minivan 0.35" and calcined river sand. Plants were adapted to hydroponic installation characterized by intensive development of shoots and leaves, which are 1.5-2 times higher than those figures have regenerated, adapted in the sand. Thus the root system formed in hydroponic conditions had roots of the second order. Before planting in the ground regenerated plants were treated with 0.1% sodium sulfate, chitosan and ultrasound. Ultrasonic irradiation was carried out using the apparatus of "Wave" with a frequency mechanical vibrations of 22±1,65 kHz. Spraying the leaves of 0.1% sodium sulfate chitosan virtually had no impact on plant growth and development. Soaking the root system in the same solution also significantly increased the number of whiskers (1.75 pcs.) and leaves (0.35 pcs.), the length of the whiskers (1.86 cm) and significantly increased the height of the outlet 1.79 cm. Treatment of plants with ultrasound for 10 minutes slightly increased the amount of whiskers (1.54 pcs.) and length (1.26 cm), and significantly increases the height of the slots (1.79 cm). Designed experiments aimed to assess the ability of reproduction of plants of strawberry produced in the traditional way and in vitro studies have shown that plants propagated in vitro formed 6,43±0,99 whiskers per socket, while the plants produced by conventional method had no whiskers.
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