SEASONAL AND AGE VARIATIONS OF BERBERINE CONTENT IN INTRODUCED BERBERIS SIBIRICA PALL.

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Нечепуренко, Иван Васильевич ; Комарова, Нина Ивановна ; Потёмкин, Олег Николаевич ; Салахутдинов, Нариман Фаридович (2014)
  • Publisher: Altai State University
  • Journal: Khimiia rastitel'nogo syr'ia (Chemistry of plant raw material) (issn: 1029-5143, eissn: 1029-5151)
  • Related identifiers: doi: 10.14258/jcprm.1401195
  • Subject: berberine; Berberis sibirica; alkaloids dynamics; introduction; HPLC. | берберин, Berberis sibirica, динамика алкалоидов, интродукция, ВЭЖХ.

The content of isoquinoline alkaloid berberine was studied by means of HPLC in the roots and the above-ground part of introduced Berberis sibirica Pall. of various ages collected during different periods of three vegetative seasons. The roots contain 3–5 times more berberine than the stems. The content of berberine in 7-year-old roots varies slightly during the season; its mean value was 1.10% and maximum content was found in July (1,16±0.03%). The roots of the age of 3 years contain fewer berberine. Berberine chloride has been preparatively isolated from the introduced roots containing 0,95% of berberine; the yield was 0,62%, which is close to the value typical for obtaining berberine chloride from wild roots.
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