DEVELOPMENT OF METHODS OF QUALITY CONTROL OF GINSENG TINCTURA

Article Russian OPEN
Куркин, Владимир Александрович ; Акушская, Алина Сергеевна (2013)
  • Publisher: Altai State University
  • Journal: Khimiia rastitel'nogo syr'ia (Chemistry of plant raw material) (issn: 1029-5143, eissn: 1029-5151)
  • Related identifiers: doi: 10.14258/jcprm.1302177
  • Subject: ginseng, Panax ginseng C.A. Meyer, tincture, saponins, thin-layer chromatography, spectrophotometry, standardization | женьшень настоящий, Panax ginseng C.A.Meyer, настойка, сапонины, тонкослойная хроматография, спектрофотометрия, стандартизация

There were developed the techniques of qualitative and quantitative analysis of ginseng tinctures (Panax ginseng C.A. Meyer). Thin-layer chromatography (in the solvent system chloroform – methanol – water, 26 : 14 : 3; detection in the visible range after reaction with phosphowolframic acid) and UV/Visible spectroscopy of native tincture (maximum of absorption at 268±2 nm) and total saponins, purified on polyamide and reacted with 70% sulphuriс acid (maximum of absorption at 280±2 nm, 400±2 nm and 526±2 nm). The method of quantitative determination of total saponins in tinctura of ginseng by means of direct spectrophotometry (analytical wave-length at 526 nm) was developed. The contents of the total saponins are varied with 0,42% to 0,61% (calculated on ginsenoside Rg1)
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