Morphological and genetics analysis of Stachys sylvatica (Lamiaceae) coenopopulations in the mountains of South Siberia

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I. E. Yamskikh ; M. G. Kutsev ; O. V. Nefedova (2016)
  • Publisher: Altay State University
  • Journal: Turczaninowia (issn: 1560-7259, eissn: 1560-7267)
  • Related identifiers: doi: 10.14258/turczaninowia.19.1.8
  • Subject: Botany | QK1-989

<p><em>Stachys sylvatica </em>is Tertiary nemoral relict from the Sout Siberian mountains. Modification and genetic variability of 11 <em>Stachys sylvatica </em>populations from South Siberian mountains were studied. Investigations revealed that <em>S. sylvatica </em>grew in coniferous (spruce, pine, Siberian stone pine, fir, and mixed forests), aspen, linden forests and floodplain habitats. Climatic area of the species is limited to an annual precipitation of 500–1250 mm, effective heat sum 1600–2050 ºC and altitude from 150 to 700 m a.s.l. In the study of the state of the <em>S. sylvatica </em>coenopopulations we determined the population size, projective cover, evaluated the morphological variability of vegetative and genera­tive characteristics. To establish the significance of differences between the average values of the same characters we used ANOVA. It was revealed that most of the morphometric characters had medium and high level of variability. The genetic variability of species we detected by RAF-PCR method (Randomly Amplified DNA Fingerprinting). The analysis of genetic variation of the <em>S. sylvatica </em>coenopopulations showed that the studied species was characterized by a very high level of intra-population polymorphism. The maximum level of genetic variability was observed in the population growing on the border of the species area. Gene pools of all studied coenopopulations have a satisfactory condition and can be reproduced by itself. All studied coenopopulations of <em>Stachys sylvatica </em>are poorly differenti­ated and do not show the tendency to the formation of new taxa. Moreover, a similar genetic structure we have seen in the coenopopulations growing not only in different parts of the area, but also in communities radically different in environmental and phytocenotic conditions. With increasing of anthropogenic impact on coenopopulations we have observed an increase of the size of the generative organs of <em>Stachys sylvatica</em>. The separation of individuals on a geographical basis was revealed based on the analysis of morphological characters of <em>S. sylvatica</em>. Investigations revealed that <em>Stachys sylvatica </em>had wide ecological amplitude and significant variability in the stressed environmental conditions (compared to other nemoral (Tertiary) relicts). It is characterized by a high level of intrapopulation genetic variability and low degree of population differentiation.</p>
  • References (11)
    11 references, page 1 of 2

    Krutovsky K. V., Politov D. V., Altukhov Y. P. et al. (1989) Geneticheskaya izmenchivost sibirskoy kedrovoy sosny P. sibirica. Soobshchenie IV. Geneticheskoe raznoobrazie i stepen geneticheskoy differenciacii mezhdu populyaciyami [Genetic variability of Siberian cedar pine P. sibirica. IV. Genetics diversity and degree of genetic differentiation between populations] // Rus. J. Genet. 25, 11: 2009-2032 [In Russian]. (Крутовский К. В., Политов Д. В., Алту- хов Ю. П. и др. Генетическая изменчивость сибирской кедровой сосны P. sibirica. Сообщение IV. Генетическое разнообразие и степень генетической дифференциации между популяциями // Генетика, 1989. Т. 25, № 11. С. 2009-2032).

    Mamaev S. A. (1972) Formy vnutrividovoy izmenchivosti drevesnykh rasteniy [Forms of intraspecies variability of woody plants]. Nauka, Moscow, 284 pp. [In Russian]. (Мамаев С. А. Формы внутривидовой изменчивости древесных растений. М.: Наука, 1972. 284 с.).

    Nikiforova O. D. (1997) Stachys L. - Chistec [Stachys L. - Stachys] // Flora Sibiriae. Nauka, Divisio Sibirica, Novosibirsk, 11: 198-201 [In Russian]. (Никифорова О. Д. Stachys L. - Чистец // Флора Сибири. - Новосибирск: Наука. Сиб. отд-е, 1997. Т. 11. С. 198-201).

    Polikarpov N. P., Tchebakova N. M. Nazimova D. I. (1986) Klimat i gornye lesa Juzhnoy Sibiri [Climate and mountain forests of southern Siberia]. Nauka, Divisio Sibirica, Novosibirsk, 224 pp. [In Russian]. (Поликарпов Н. П., Чебакова Н. М., Назимова Д. И. Климат и горные леса Южной Сибири. Новосибирск: Наука, Сиб. отд-е, 1986. 224 с.).

    Popov M. G. (1959) Flora Sredney Sibiri [Flora of Central Siberia]. Academy of sciences press, Moscow-Leningrad, 2: 559-918 [In Russian]. (Попов М. Г. Флора Средней Сибири. М.-Л.: изд-во АН СССР, 1959. Т. 2. С. 559-918).

    Miller M. P. (1997) Tools for population genetic analyses (TFPGA) 1.3: A Windows program for the analysis of allozyme and molecular population genetic data / Northern Arizona University. 30 pp.

    Nei M. (1978) Estimation of average heterozygosity and genetic distance from a small number of individuals // Genetics 89: 538-590.

    Reynolds J., Weir B. S., Cockerham C. C. (1983) Estimation of the coancestry coefficient: Basis for a short-term genetic distance // Genetics 105: 767-779.

    Waldron J., Peace C., Searle I. et al. (2002) Randomly amplified DNA fingerprinting: a culmination of DNA marker technologies based on arbitrarily-primed PCR amplification // Journal of biomedicine and biotechnology 2(3): 141-150.

    Ward J. H. Jr. (1963) Hierarchical Grouping to Optimize an Objective Function // Journal of the American Statistical Association 58: 236-244.

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