THE STUDY OF INTRASPECIFIC VARIATION OF OREGANO (ORIGANUM VULGARE L.) ESSENTIAL OIL

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Хазиева (Khazieva), Фирдаус (Firdaus) Мухаметовна (Mukhametovna) ; Осипов (Osipov), Владимир (Vladimir) Ионович (Ionovich) ; Коротких (Korotkikh), Ирина (Irina) Николаевна (Nikolaevna) (2016)
  • Publisher: Altai State University
  • Journal: Khimiia rastitel'nogo syr'ia (Chemistry of plant raw material) (issn: 1029-5143, eissn: 1029-5151)
  • Related identifiers: doi: 10.14258/jcprm.2016041178
  • Subject: Origanum vulgare; essential oil; chemotypes; gas-liquid chromatography; mass-spectrometry | душица обыкновенная; эфирное масло; хемотипы; газожидкостная хроматография; масс-спектрометрия

The intraspecies variability of essential oil (EO) compounds in the Oregano plants (Origanum vulgare L.) was studied. To this end, total content of EO, as well as the composition and content of EO compounds were analyzed in the samples of three varieties and six clones of Oregano plants obtained in the course of breeding, and which were distinguished by some morphological characteristics (height and form of plant, flowers coloring). Application of gas-liquid chromatography with mass spectrometric detector (GC-MS) allowed determining in the samples of Oregano EO 120 individual compounds, 60 of which were identified. The major compounds were terpinene-4-ol (16,4%), β-caryophyllene (12,4%), germacrene-D (9,7%), caryophyllene oxide (8,2%), spathulenol (7,7%), α-cadinol (6,0%), γ-terpinen (2,9%), α-cadinol (2,8%), α-humulene (2,2%), δ-cadinene (1,9%) и α-terpineol (1,7%). They account for 71,9 % of total content of EO. Analysis of GC-MS data by the methods of multivariate statistics show that the studied clones (varieties) of Oregano plants are divided on the two groups with high and low EO content. Compounds that were mainly responsible for the differences of these two groups in the EO content were determined. It is assumed the existence of a positive relationship between high EO content and compact, dwarf form of Oregano plants.
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