publication . Article . 2017

Pro-embrionary Somatic Structure of Three Cacao Genotypes (Theobroma Cacao l.) Using Staminodes

Garate-Navarro, Mar Asunción; Arévalo-Gardini, Enrique; Correa do Bomfim-Costa, Larissa; da Costa-Silva, Delmira;
Open Access English
  • Published: 07 Jul 2017 Journal: International Annals of Science (issn: 2456-7132, eissn: 2456-7132, Copyright policy)
  • Publisher: AIJR Publisher
Staminodes of three cocoa genotypes (BT1, ICS-39 and CCN-51), were used to perform the characterization of somatic embryos induced and maintained by DKW culture medium enriched with 2% of sucrose, 10% of coconut water and 0.01% of cysteine, 2,5 mg/l 2,4 D, 5 µg/lTDZ respectively. The ANOVA and the comparison mean test, shown significant differences between globular-, heart-, torpedo- and cotyledonal-stages for each genotype. Additionally, the embryogenic stages and regenerants plants proportions were highest for Blanco Tarapotino 1 (BT1) genotype. A morphological comparison of somatic and zygotic embryos shows similarities in form and symmetry.
Medical Subject Headings: food and beverages
free text keywords: Tissue culture, tropical crops, in vitro propagation, micro propagation
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