publication . Article . 2017

Pro-embrionary Somatic Structure of Three Cacao Genotypes (Theobroma Cacao l.) Using Staminodes

Garate-Navarro, Mar Asunción; Arévalo-Gardini, Enrique; Correa do Bomfim-Costa, Larissa; da Costa-Silva, Delmira;
Open Access English
  • Published: 07 Jul 2017 Journal: International Annals of Science (issn: 2456-7132, eissn: 2456-7132, Copyright policy)
  • Publisher: AIJR Publisher
Abstract
Staminodes of three cocoa genotypes (BT1, ICS-39 and CCN-51), were used to perform the characterization of somatic embryos induced and maintained by DKW culture medium enriched with 2% of sucrose, 10% of coconut water and 0.01% of cysteine, 2,5 mg/l 2,4 D, 5 µg/lTDZ respectively. The ANOVA and the comparison mean test, shown significant differences between globular-, heart-, torpedo- and cotyledonal-stages for each genotype. Additionally, the embryogenic stages and regenerants plants proportions were highest for Blanco Tarapotino 1 (BT1) genotype. A morphological comparison of somatic and zygotic embryos shows similarities in form and symmetry.
Subjects
Medical Subject Headings: food and beverages
free text keywords: Tissue culture, tropical crops, in vitro propagation, micro propagation
Download from
18 references, page 1 of 2

J. Cuatrecasas, Cacao and its allies: a taxonomic revision of the genus Theobroma. Contribution from the United States National Herbarium, Washington, v. 35, p. 379- 614. 1964.

British. Journal Nutrition, v. 99, p. 1-11. 2007.

Netherlands: Kluwer Academic Publisher, v.2. p.291- 310. 1995.

Z. Li, A. Traore, S. Maximova, M.J. Guiltinan, Somatic embryogenesis and plant regeneration from floral explants of cacao (Theobroma cacao L.) using thidiazuron. In Vitro Cellular and Developmental Biology - Plant, v.34, p.293-299, 1998..

Zeitschrift für Pflanzenphysiologie, Stuttgart, v. 113, n.

4, p. 347-358, 1984.

Agril. Biol Sci, v. 6, p. 31-43. 1988.

Traoré, M.J. Guiltinan, Efficiency, genotypic variability, and cellular origin of primary and secondary somatic embryogenesis of Theobroma cacao L. In Vitro Cellular & Developmental Biology, v. 38, p. 252-259. 2002.

C.L. Tan, D.B. Furtek,. Development of an in vitro regeneration system for Theobroma cacao from mature tissues. Plant Science, v. 164, p. 407-412, 2003. [OpenAIRE]

E.B. Esan, Tissue culture studies on cacao (Theobroma cacao L.): a supplementation of current research. In: INTERNATIONAL COCOA RESEARCH CONFERENCE, v. 5, p.116-125. 1997.

V.C. Pence, P.M. Hasegawa, J. Janick, Asexual Embryogenesis in Theobroma cacao L. Journal of American Society for Horticultural Science, v. 104, p.

19, p. 839-841. 1984.

F.J. Novak, G. Owusu, Somatic Embryogenesis and In Vitro Plant Development of Cocoa (Theobroma cacao L.). Nuclear techniques and in vitro culture for plant improvement. IAEA. Vienna, v. 17, p. 443- 449. 1986.

Y. Adu-Ampomah, F.J. Novak, R. Afza, M. Van Duren and M. Perea-Dallos. Initiation and growth of somatic [15] [17] embryos of cocoa (Theobroma cacao L.). Cafe, Cacao Thé, v. 32, p.187-197, 1988.

D. Omokolo Ndoumou, G. Tsala Ndzomo and N. Niemenak, Phenol Content, Acidic Peroxidase and IAA- oxidase During Somatic Embryogenesis in Theobroma cacao L. Biologia Plantarum, v. 39, p.337- 347. 1997. [OpenAIRE]

18 references, page 1 of 2
Powered by OpenAIRE Research Graph
Any information missing or wrong?Report an Issue