publication . Article . 2019

Symptom Severity of Nicotiana benthamiana Plants Inoculated with Agrobacterium Containing Infectious DNA-A Clones of Honeysuckle Yellow Vein Virus (HYVV)

Oh, Sung; Choi, Chang Won;
Open Access English
  • Published: 17 Apr 2019 Journal: International Annals of Science (issn: 2456-7132, eissn: 2456-7132, Copyright policy)
  • Publisher: AIJR Publisher
Abstract
To investigate the pathogenicity and virulence of the Honeysuckle yellow vein virus (HYVV) lacking betasatellites, PCR amplified unit-lengths of DNA-A genome of HYVV-[DJ] were cloned into binary vector pRI101-AN, and generated HYVV-[DJ]-1mer, -1.3mer and -2mer genomes. Each construct was transformed into Agrobacterium cells and agro-inoculated into young leaves of Nicotiana benthamiana. Except for the HYVV-[DJ]-1mer, HYVV-[DJ]-1.3mer and -2mer clones caused pronounced disease symptoms in N. benthamiana. HYVV-[DJ]-2mer agro-inoculated plants showed more severe plant stunting with downward leaf curling and crinkling than those of HYVV-[DJ]-1.3mer agro-inoculated p...
Subjects
Medical Subject Headings: food and beveragesfungitechnology, industry, and agriculture
free text keywords: Honeysuckle yellow vein virus (HYVV), Symptom severity, Virulence of HYVV DNA-A clones, V1 protein gene, SYBR Green-based real-time PCR
Download from
Powered by OpenAIRE Research Graph
Any information missing or wrong?Report an Issue