project . 2009 - 2016 . Closed

PI3K signalling; the rules of engagement.

Wellcome Trust
  • Funder: Wellcome TrustProject code: 085889
  • Funded under: Cellular and Molecular Neuroscience Funder Contribution: 1,277,010 GBP
  • Status: Closed
  • Start Date
    19 Jan 2009
    End Date
    18 Jan 2016
Description
To use, PI3Kalpha-manipulated (conditional-KO and kinase-dead knock-in) mice and PI3Kalpha-selective, small-molecule inhibitors to define both the redundant and unique roles for PI3Kalpha in neutrophils. With our unpublished work on PI3Ksdelta and beta this will allow us to assemble the first integrated view of Class IA PI3K signalling in a specific cellular context. To establish which domains in the Class IA PI3Ks are critical to function in vivo; by expressing wild-type, point-mutated and chimeric-(carrying single domains switched with other members of the sub-family)-PI3Ks in PI3K-KO haemopoeitic stem cells and assessing their ability to rescue the establishe...
Description
To use, PI3Kalpha-manipulated (conditional-KO and kinase-dead knock-in) mice and PI3Kalpha-selective, small-molecule inhibitors to define both the redundant and unique roles for PI3Kalpha in neutrophils. With our unpublished work on PI3Ksdelta and beta this will allow us to assemble the first integrated view of Class IA PI3K signalling in a specific cellular context. To establish which domains in the Class IA PI3Ks are critical to function in vivo; by expressing wild-type, point-mutated and chimeric-(carrying single domains switched with other members of the sub-family)-PI3Ks in PI3K-KO haemopoeitic stem cells and assessing their ability to rescue the establishe...
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