project . 2019 - 2021 . Closed

MitoScaling

Mitotic scaling to cell size diversity during vertebrate embryonic development
Open Access mandate for Publications and Research DataOpen Access mandate for ... European Commission
  • Funder: European CommissionProject code: 792175 Call for proposal: H2020-MSCA-IF-2017
  • Funded under: H2020 | MSCA-IF-EF-ST Overall Budget: 187,420 EURFunder Contribution: 187,420 EUR
  • Status: Closed
  • Start Date
    01 Sep 2019
    End Date
    31 Aug 2021
  • Detailed project information (CORDIS)
Description
Cell division is a common process to all cell types in a multicellular organism. During mitosis, equal chromosome segregation in anaphase is regulated by an Aurora B phosphorylation gradient, centered at the midplane between the two chromosome sets. The gradient gives positional information that allows nuclear envelope reformation (NER) only when chromosomes are far enough from the kinase activity. Considering the 120μm human zygote and a 15μm fibroblast, how can the gradient scale with cell size over such wide range of sizes? To unravel the scaling mechanism, I will measure the biophysical parameters of this phosphorylation gradient using a FRET sensor and opto...
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Description
Cell division is a common process to all cell types in a multicellular organism. During mitosis, equal chromosome segregation in anaphase is regulated by an Aurora B phosphorylation gradient, centered at the midplane between the two chromosome sets. The gradient gives positional information that allows nuclear envelope reformation (NER) only when chromosomes are far enough from the kinase activity. Considering the 120μm human zygote and a 15μm fibroblast, how can the gradient scale with cell size over such wide range of sizes? To unravel the scaling mechanism, I will measure the biophysical parameters of this phosphorylation gradient using a FRET sensor and opto...
Partners
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