The overarching objective of UNISECO is to strengthen the sustainability of EU farming systems, through co-constructing practice-validated strategies and incentives for the promotion of improved agro-ecological approaches. We will enhance the understanding of socio-economic and policy drivers and barriers for further development and implementation of agro-ecological approaches in EU farming systems to identify and facilitate more effective management strategies for European agriculture. This requires a wider systems perspective, considering socio-economic, ecological and political settings at a territorial level. We will operationalise a socio-ecological systems framework that integrates external settings into a sustainability assessment of farming systems, paying particular attention to the role of different types of actors and their roles in sub-systems. UNISECO will provide a methodological toolkit to assess the environmental, economic and social impacts of innovative strategies and incentives for agro-ecological approaches in EU farming systems at farm and territorial levels. The methodological toolkit will be tested in participatory case studies in 15 European countries with different biophysical, socio-economic and socio-cultural contexts of farming systems. UNISECO is committed to a genuine transdisciplinary process, reflected in consortium composition of stakeholder and scientific partners, and the emphasis on multi-actor engagement. Group learning processes, integrating practical and theoretical knowledge, will benefit scientists, stakeholders and end-users through improved understanding on how to strengthen sustainability of EU farming systems. UNISECO addresses the work programme topic SFS-29-2017 and contributes to Sustainable Food Security by identifying and supporting farming systems that enable the production of healthy food while preserving the environment and bringing added value to farm households and the different stakeholders of the value chain.