Some Secrets of Fluorescent Proteins: Distinct Bleaching in Various Mounting Fluids and Photoactivation of Cyan Fluorescent Proteins at YFP-Excitation

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Malkani, Naila ; A. Schmid, Johannes
  • Publisher: Figshare
  • Related identifiers: doi: 10.1371/journal.pone.0018586
  • Subject: yfp-excitation | bleaching | secrets | cyan | fluorescent | Cell Biology | photoactivation | mounting | proteins | Biochemistry | Biophysics | fluids
    mesheuropmc: macromolecular substances

<div><h3>Background</h3><p>The use of spectrally distinct variants of green fluorescent protein (GFP) such as cyan or yellow mutants (CFP and YFP, respectively) is very common in all different fields of life sciences, e.g. for marking specific proteins or cells or to de... View more
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